ENDOGLUS-RO
Roche
Endoproteinase Glu-C Sequencing Grade
from Staphylococcus aureus V8
Synonyma:
protease
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
Doporučené produkty
biological source
Staphylococcus aureus (V8)
form
lyophilized (salt-free)
lyophilized
specific activity
≥15 units/mg protein
mol wt
Mr 27 kDa
packaging
pkg of 3 × 50 μg (11047817001)
pkg of 50 μg (11420399001)
manufacturer/tradename
Roche
optimum pH
4.0-7.8
storage temp.
2-8°C
Související kategorie
General description
Endoproteinase Glu-C Sequencing Grade is a serine protease isolated from Staphylococcus aureus V8 as a highly purified and specific protease. Inhibitors of Endoproteinase Glu-C are DFP, α2-macroglobulin and TLCK.
Specificity
Serine protease that very specifically hydrolyzes peptide bonds at the carboxylic side of Glu (in ammonium bicarbonate, pH 7.8, or ammonium acetate buffer, pH 4.0), or Glu and Asp (in phosphate buffer, pH 7.8). The specificity of Endoproteinase Glu-C Sequencing Grade is verified with the oxidized B-chain of insulin (insulin Box) as substrate. High concentrations of Endoproteinase Glu-C Sequencing Grade (1 part by weight enzyme with 10 parts by weight insulin Box) are incubated to detect traces of impurities, for example, contaminating proteases.
Heat inactivation: Most proteases sequencing grade are stopped by boiling for 5 minutes.
Heat inactivation: Most proteases sequencing grade are stopped by boiling for 5 minutes.
Application
Use Endoproteinase Glu-C Sequencing Grade for protein structure analysis and for sequence analysis.
Features and Benefits
Free of impurities that may interfere with separation of peptides in RP HPLC.
Preparation Note
Working concentration: 1/100 to 1/20 (w/w)
With a minimum amount of 1 to 5 μg insulin Box, sufficient levels can be detected using the Column Aquapore RUP 300, 4.6 x 100 nm, 7 μm.
Working solution: Solvent is recommended in double-distilled water.
Solvents other than water have not been tested; Roche recommends using 50 mM Hepes, pH 7.8.
Storage conditions (working solution): +2°C to +8°C for 1-2 days.
Stability: At +2 to +8°C within specification range for 12 months; stored dry.
With a minimum amount of 1 to 5 μg insulin Box, sufficient levels can be detected using the Column Aquapore RUP 300, 4.6 x 100 nm, 7 μm.
Working solution: Solvent is recommended in double-distilled water.
Solvents other than water have not been tested; Roche recommends using 50 mM Hepes, pH 7.8.
Storage conditions (working solution): +2°C to +8°C for 1-2 days.
Stability: At +2 to +8°C within specification range for 12 months; stored dry.
Reconstitution
Lyophilized Endoproteinase Glu-C sequencing grade is reconstituted in double-dist. water. To avoid autolysis, the incubation temperature should not exceed 25 °C.
Other Notes
For life science research only. Not for use in diagnostic procedures.
signalword
Danger
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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