1.16883

Fractogel^® EMD DEAE (M)

• Synonyma: Fractogel^® EMD DEAE (M)
• UNSPSC Code: 41115711

Vlastnosti

• Quality Level: 400
• ligand: diethylaminoethyl
• description: weak anion exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
• sterility: sterile (Caustic Stable)
• product line: Fractogel^®
• form: resin
• parameter: 170 cm/hr flow rate; 8 bar max. pressure
• matrix active group: methacrylate
• mean particle size: 40-90 μm
• capacity: 100 mg binding capacity (BSA/mg of resin)
• transition temp: flash point 35 °C (calculated)
• density: 1.430 g/cm³
• bulk density: 1000 kg/m³
• application(s): gene therapy; mAb; vaccine development
• separation technique: weak anion exchange
• storage temp.: 2-30°C

Popis

General description

Weak anion exchange Fractogel^® resin featuring the tentacle technology, for purification of acidic and neutral proteins and peptides from multiple sources, pDNA purification, DNA, RNA, and endotoxin removal, large viruses purification, vaccine purification, blood fractionation, and more

Features and Benefits

Fractogel^® EMD DEAE (M) enables:

• Excellent binding to large viruses and plasmid DNA
• Homogenous binding with high selectivity and purity
• Lower elution volumes for the highest purity levels
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the degree of the ionization of the functional groups of the exchanger
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Packaging

• 1.16883.0100: Fractogel^® EMD DEAE (M) Resin 100ml
• 1.16883.0010: Fractogel^® EMD DEAE (M) Resin 10ml
• 1.16883.0500: Fractogel^® EMD DEAE (M) Resin 500ml
• 1.16883.5000: Fractogel^® EMD DEAE (M) Resin 5L

Plastic bottle

Analysis Note

Appearance: Milky turbid suspension,free from impurities (foreign particles)
Microscopic evaluation: Uniform spherical particles,no agglomerates,no fines
Extractable matter (water): ≤ 0.03 %
Cerium: ≤ 1 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (bovine serum albumin): 80 - 120 mg/ml
Functional test (b:a): ≤ 0.25
Functional test: Separation of conalbumin and human serum albumin

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class: 3 - Flammable liquids
• wgk_germany: WGK 3
• flash_point_f: 95.0 °F
• flash_point_c: 35 °C