The Osteomoll instructions recommend using a needle to check for demineralization of calcium in tissue, but a more accurate method involves a chemical end point determination to ascertain the absence of calcium in the tissue. Tissues can also be examined by X-ray to detect the presence of calcium. A cost-effective chemical method involves using 5 ml of the used decalcifying solution and neutralizing it with 5 ml of concentrated ammonium hydroxide. Litmus paper can be used to verify a neutral pH. Then, 5 ml of saturated ammonium oxalate is added, and the solution is allowed to stand for 30 minutes. If the solution is turbid after 30 minutes, it indicates that calcium (calcium oxalate) is still present in the tissue. The process of decalcification should be continued, periodically checking for completion. Smaller tissue pieces should be checked more frequently, while it is unnecessary to check larger tissue pieces as frequently.
1.01728
OSTEOSOFT®
mild decalcifier-solution for histology
Vybrat velikost
1 880,00 Kč
13 000,00 Kč
O této položce
Přeskočit na
Název produktu
OSTEOSOFT®, mild decalcifier-solution for histology
form
liquid
IVD
for in vitro diagnostic use
color
dark red
pH
7.0-7.3 (20 °C in H2O)
density
1.10 g/mL at 20 °C
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
15-25°C
Quality Level
1 of 4
Tato položka | 1.01736 | 1.00496 | 1.08000 |
|---|---|---|---|
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| form liquid | form liquid | form liquid | form liquid |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) clinical testing |
| density 1.10 g/mL at 20 °C | density 1.10 g/mL at 20 °C | density 1.017 g/cm3 at 20 °C | density 1.18 g/cm3 at 20 °C |
| pH 7.0-7.3 (20 °C in H2O) | pH <1 (20 °C in H2O) | pH 6.7-7.1 ( in H2O) | pH - |
| IVD for in vitro diagnostic use | IVD for in vitro diagnostic use | IVD for in vitro diagnostic use | IVD for in vitro diagnostic use |
General description
Decalcification is necessary for optical microscopic examinations of hard tissue in routine histological procedures. For the decalcification of sensitive, calcium-containing tissues, such as iliac crest biopsies, OSTEOSOFT® can be used. Its formulation is based on EDTA. Its pendant, OSTEOMOLL®(Product number 1.01736), can be used for the decalcification of hard tissue such as teeth and bone as its formulation is based on formaldehyde and acetic acid. The amount of OSTEOSOFT®needed is dependent upon on the size, type and thickness of the material. Both solutions are color-coded, to distinguish them from each other, fixatives and solvents used in the histological laboratory. OSTEOSOFT® is color-coded yellow, OSTEOMOLL® is color-coded blue. Whereby OSTEOSOFT® preserves the antigen structures in the tissue, thus, an IHC analysis can be performed, OSTEOMOLL® will be used for tissues that are not destined for antibody analysis.
Both decalcifiers can be used for clinical diagnostic purposes and in laboratory accreditation processes as they are certified and registered as IVD and CE product. OSTEOSOFT® is used only once and fresh solution must be used for each tissue. The quantity of OSTEOSOFT® required is dependent on the size, type, and density of the respective material. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Legal Information
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - STOT RE 2 Inhalation
target_organs
Respiratory Tract
Skladovací třída
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Osvědčení o analýze (COA)
Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.
Již tento produkt vlastníte?
Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
Související obsah
Seznamte se s kritickým významem barvení biologických tkání pro výzkum a klinickou patologii pomocí standardních a speciálních barviv.
Learn about the criticality of biological tissue staining for research and clinical pathology using standard and special stains and dyes.
-
What is the method for determining when decalcification is complete when using a decalcification solution, such as Osteosoft, Osteomoll, or Decalcifying Solution Light?
1 answer-
Helpful?
-
Active Filters
Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..
Obraťte se na technický servis