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Merck

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
RESUMEN

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

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Sigma-Aldrich
Metanol, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Acetona, ACS reagent, ≥99.5%
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Tetrahidrofuran, inhibitor-free, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Metanol, ACS reagent, ≥99.8%
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Hidróxido de amonio solution, ACS reagent, 28.0-30.0% NH3 basis
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Acetona, suitable for HPLC, ≥99.9%
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N,N-Dimetilformamida, ACS reagent, ≥99.8%
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N,N-Dimetilformamida, suitable for HPLC, ≥99.9%
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Metanol, suitable for HPLC, gradient grade, ≥99.9%
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Cloroformo, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
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Acetona, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
Sigma-Aldrich
Tolueno, ACS reagent, ≥99.5%
Sigma-Aldrich
Tetrahidrofuran, contains 250 ppm BHT as inhibitor, ACS reagent, ≥99.0%
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Dietiléter, anhydrous, ACS reagent, ≥99.0%, contains BHT as inhibitor
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Pyridine, ACS reagent, ≥99.0%
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Metanol, HPLC Plus, ≥99.9%
Sigma-Aldrich
Cloroformo, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer
Sigma-Aldrich
Dietiléter, suitable for HPLC, ≥99.9%, inhibitor-free
Sigma-Aldrich
Cloroformo, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
Tolueno, suitable for HPLC, 99.9%
Sigma-Aldrich
Tolueno, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
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Dietiléter, ACS reagent, anhydrous, ≥99.0%, contains BHT as inhibitor
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Acetona, Laboratory Reagent, ≥99.5%
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2- Mercaptoetanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
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Hidróxido de amonio solution, 28% NH3 in H2O, ≥99.99% trace metals basis
Sigma-Aldrich
Cloroformo, contains ethanol as stabilizer, ACS reagent, ≥99.8%
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2- Mercaptoetanol, ≥99.0%
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Formaldehído solution, for molecular biology, 36.5-38% in H2O
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Cloruro de sodio, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
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Pyridine, suitable for HPLC, ≥99.9%