SAE0046

Carboxypeptidase A from bovine pancreas

(Type II-PMSF treated), ≥20 units/mg protein

• Sinónimos: Carboxypeptidase A, Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase
• Número de CAS: 11075-17-5
• Comisión internacional de enzimas: 3.4.17.1 (BRENDA, IUBMB)
• Número MDL: MFCD00130718
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: bovine pancreas
• Nivel de calidad: 200
• Formulario: aqueous suspension
• calidad: (Type II-PMSF treated)
• actividad específica: ≥20 units/mg protein
• mol peso: ~35 kDa
• purificado por: 2× crystallization
• impurezas: ≤0.05 BTEE units/mg protein chymotrpsin; ≤10 BAEE units/mg protein trypsin
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Carboxypeptidase A (CPA) is a secreted protease is liberated after the activation of mast cells to facilitate acute anaphylaxis. Carboxypeptidase A has a long half-life in vivo, when compared to other secreted proteases.

Aplicación

Carboxypeptidase A from bovine pancreas has been used in in vitro simulated digestion.

Carboxypeptidase A from bovine pancreas has been used in a study to investigate the expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli. Carboxypeptidase A from bovine pancreas has also been used in a study to investigate the isolation and partial characterization of precursor forms of ostrich carboxypeptidase.

Acciones bioquímicas o fisiológicas

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.†

Definición de unidad

One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.

Nota de preparación

Treated with phenylmethylsulfonyl fluoride to eliminate trypsin and chymotrypsin activity. Dialyzed and recrystallized: aqueous suspension with toluene added.

Nota de análisis

Protein determined by E1%/278

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable