E2257

β-Estradiol

powder, γ-irradiated, suitable for cell culture

• Sinónimos: 1,3,5-Estratriene-3,17β-diol, 17β-Estradiol, 3,17β-Dihydroxy-1,3,5(10)-estratriene, Dihydrofolliculin
• Fórmula empírica (notación de Hill): C₁₈H₂₄O₂
• Número de CAS: 50-28-2
• Peso molecular: 272.38
• Beilstein: 1914275
• Número CE: 200-023-8
• Número MDL: MFCD00003693
• Código UNSPSC: 12352209
• ID de la sustancia en PubChem: 24894462
• NACRES: NA.77

Propiedades

• origen biológico: synthetic (organic)
• Nivel de calidad: 200
• esterilidad: γ-irradiated
• Línea del producto: BioXtra
• Ensayo: ≥98% (HPLC)
• Formulario: powder
• técnicas: cell culture | mammalian: suitable
• mp: 176-180 °C (lit.)
• solubilidad: ethanol: 1 mg/mL, clear, colorless to very faintly yellow
• idoneidad: suitable for
• Condiciones de envío: ambient
• temp. de almacenamiento: room temp
• cadena SMILES: O[C@H]1CC[C@@]2([H])[C@]3([H])CCC4=CC(O)=CC=C4[C@@]3([H])CC[C@@]21C
• InChI: 1S/C18H24O2/c1-18-9-8-14-13-5-3-12(19)10-11(13)2-4-15(14)16(18)6-7-17(18)20/h3,5,10,14-17,19-20H,2,4,6-9H2,1H3/t14-,15-,16+,17+,18+/m1/s1
• Clave InChI: VOXZDWNPVJITMN-ZBRFXRBCSA-N
• Información sobre el gen: human ... ESR1(2099) , ESR2(2100) , ESRRA(2101) , ESRRB(2103) , GPER(2852) , SERPINA6(866) ; mouse ... Esr1(13982) , Esr2(13983) , Esrra(26379) ; rat ... Afp(24177) , Ar(24208) , Esr1(24890) , Esr2(25149) , Shbg(24775)

Descripción

Descripción general

β-estradiol is a natural human estrogen. It is important for the growth of breast and reproductive epithelia, maturation of long bones and the development of secondary sexual characteristics. Estradiol is produced by immature germ cells, spermatozoa, Leydig cells and Sertoli cells. It plays a crucial role in proliferation, regulation of ion transport and regulation of apoptosis in Sertoli cells. Estradiol regulates sperm concentration, motility and morphology. β-estradiol functions as a menopausal hormone. It is implicated in replacement therapy for female hypogonadism or primary ovarian failure. Reduced levels of β-estradiol are associated with heart disease and osteoporosis.

Aplicación

β-Estradiol is used to study cell differentiation and transformations (tumorigenicity).

β-estradiol has been used:

• for primary culture of mouse endometrial stromal cells (ESCs)
• for estrogen treatment in vitro
• in the in vitro maturation of cumulus–oocyte complexes (COCs)

Acciones bioquímicas o fisiológicas

The major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female phenotype in embryogenesis and during puberty by regulating gene transcription and, thus, protein synthesis. It also induces the production of gonadotropins which, in turn, induce ovulation. Exposure to estradiol increases breast cancer incidence and proliferation.

The major estrogen secreted by the premenopausal ovary.

Forma física

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw

Reconstitución

To prepare 20 μg/ml stock solution: add 1 ml absolute ethanol; gently swirl to dissolve; add 49 ml sterile medium while mixing.

Información de seguridad

• Pictogramas: GHS08,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H351,H360FD,H362,H410
• Consejos de prudencia: P202 - P260 - P263 - P264 - P273 - P308 + P313
• Clasificaciones de peligro: Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Lact. - Repr. 1A
• Código de clase de almacenamiento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges