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11633716001

Roche

Anti-Digoxigenin-POD (poly), Fab fragments

from sheep

Sinónimos:

anti-digoxigenin, digoxigenin

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About This Item

Código UNSPSC:
12352203

origen biológico

sheep

Nivel de calidad

100

conjugado

peroxidase conjugate

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

polyclonal

Formulario

lyophilized

envase

pkg of 50 U

fabricante / nombre comercial

Roche

isotipo

IgG

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to polymerized horseradish peroxidase.

Especificidad

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

Aplicación

Anti-Digoxigenin-POD (poly), Fab fragments has been used for the detection of digoxigenin-labeled compounds using:
  • whole-mount in situ hybridization
  • fluorescence in situ hybridization
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • Southern blot
  • western blot
  • double labeling experiments
  • lectin blots

Anti-Digoxigenin-POD (poly), Fab fragments give considerably higher signal-to-noise values compared to anti-Digoxigenin-Fab fragment conjugated to the unpolymerized horseradish peroxidase in ELISA applications. It is specifically useful when high sensitivity is required.
Anti-Digoxigenin-POD (poly), Fab fragments have not been evaluated in immunohistochemistry.

Nota de preparación

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
  • Dot blot: 50 to 100 mU/ml
  • ELISA: 2 to 50 mU/ml
  • Western blot: 50 to 100 mU/ml
  • Southern blot: 50 to 100 mU/ml

Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

Reconstitución

Add 1 ml double-distilled water to a final concentration of 50 U/ml.

Otras notas

For life science research only. Not for use in diagnostic procedures.

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Pictogramas

Exclamation mark
GHS07

Palabra de señalización

Warning

Frases de peligro

H317

Clasificaciones de peligro

Skin Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

does not flash

Punto de inflamabilidad (°C)

does not flash

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Certificados de análisis (COA)

Lot/Batch Number
75950500
60789500
56791000
54733800
38130400

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The ability to determine spatial and temporal microRNA (miRNA) accumulation at the tissue, cell and subcellular levels is essential for understanding the biological roles of miRNAs and miRNA-associated gene regulatory networks. This protocol describes a method for fast and effective
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Antibody interactions with Fcγ receptors (FcγRs), like FcγRIIIA, play a critical role in mediating antibody effector functions and thereby contribute significantly to the biologic and therapeutic activity of antibodies. Over the past decade, considerable work has been directed towards production
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