11685619910
Roche
Fluorescein RNA Labeling Mix
solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization
Sinónimos:
RNA labeling
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
41105500
Productos recomendados
form
solution
Quality Level
usage
sufficient for 20 reactions
packaging
pkg of 40 μL
manufacturer/tradename
Roche
technique(s)
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
storage temp.
−20°C
General description
Fluorescein RNA Labeling Mix is a convenient nucleotide mixture for the labeling of RNA with fluorescein-12-uridine triphosphate (UTP).
Specificity
Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0).
Application
Fluorescein-labeled RNA has been used in a variety of hybridization techniques such as:
- in situ hybridization to tissues
- Southern blots
- northern blots
- plaque or colony lifts
- RNase protection experiments
Quality
The mix is function tested in combination with the DIG RNA Labeling Kit.
Principle
Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer.
Preparation Note
10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C).
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
No data available
flash_point_c
No data available
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Zhimin Jiang et al.
Nature microbiology, 6(7), 932-945 (2021-05-15)
The retinoic acid-inducible gene I (RIG-I) receptor senses cytoplasmic viral RNA and activates type I interferons (IFN-I) and downstream antiviral immune responses. How RIG-I binds to viral RNA and how its activation is regulated remains unclear. Here, using IFI16 knockout
Lucia Caceres et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 248(12), 1243-1256 (2019-10-01)
Familial exudative vitreoretinopathy (FEVR) is a rare congenital disorder characterized by a lack of blood vessel growth to the periphery of the retina with secondary fibrovascular proliferation at the vascular-avascular junction. These structurally abnormal vessels cause leakage and hemorrhage, while
Yasufumi Hayano et al.
Science advances, 7(29) (2021-07-16)
The most prominent structural hallmark of the mammalian neocortical circuitry is the layer-based organization of specific cell types and synaptic inputs. Accordingly, cortical inhibitory interneurons (INs), which shape local network activity, exhibit subtype-specific laminar specificity of synaptic outputs. However, the
Anke J Roelofs et al.
Annals of the rheumatic diseases, 79(12), 1625-1634 (2020-09-24)
Osteophytes are highly prevalent in osteoarthritis (OA) and are associated with pain and functional disability. These pathological outgrowths of cartilage and bone typically form at the junction of articular cartilage, periosteum and synovium. The aim of this study was to
Cheng-Hai Zhang et al.
Communications biology, 4(1), 332-332 (2021-03-14)
A hallmark of cells comprising the superficial zone of articular cartilage is their expression of lubricin, encoded by the Prg4 gene, that lubricates the joint and protects against the development of arthritis. Here, we identify Creb5 as a transcription factor
Artículos
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico