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11207741910

Roche

Anti-Digoxigenin-Fluorescein, Fab fragments

from sheep

Sinónimos:

anti-digoxigenin, digoxigenin

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About This Item

Código UNSPSC:
12352203

origen biológico

sheep

Nivel de calidad

100

conjugado

fluorescein conjugate

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

polyclonal

Formulario

lyophilized

envase

pkg of 200 μg

fabricante / nombre comercial

Roche

isotipo

IgG

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

Anti-Digoxigenin-Fluorescein, Fab fragments has fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to fluorescein.

Especificidad

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

Aplicación

Anti-Digoxigenin-Fluorescein, Fab fragments are used for the detection of digoxigenin-labeled compounds using:
  • Digoxigenin-labeled sugars in glycoconjugate research
  • Fluorescent in situ hybridization (FISH)
  • Immunohistocytochemistry
  • In situ hybridization
Anti-Digoxigenin-Fluorescein, Fab fragments cannot be used for membrane applications.

Nota de preparación

  • Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:Detection of digoxigenin-labeled sugars in glycoproteins: 50 to 20μg/ml
  • Fluorescent in situ hybridization (FISH): 1 to 20μg/ml
  • Immunohistocytochemistry: 20 to 50μg/ml
  • In situ hybridization: 20 to 50μg/ml

The anti-DIG anitbody is highly specific and sensitive. Therefore, for FISH, a concentration of 1μg/ml is sufficient, and only in case of critical probes, this concentration may be raised to <10μg/ml (with a distinct background to be expected).
Working solution: Reconstitution
Add 1 ml double-distilled water to a final concentration of 200μg/ml.

Blocking:
PBS, 0.5% bovine serum albumin (w/v), pH 7.4.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore, pH can be increased up to pH 8.5 to 9.0.
Storage conditions (working solution): Always prepare fresh!
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.

Reconstitución

Add 1 ml double-distilled water to a final concentration of 200 μg/ml.

Otras notas

For life science research only. Not for use in diagnostic procedures.

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Pictogramas

Exclamation mark
GHS07

Palabra de señalización

Warning

Frases de peligro

H317,H412

Clasificaciones de peligro

Aquatic Chronic 3 - Skin Sens. 1

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

does not flash

Punto de inflamabilidad (°C)

does not flash

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Certificados de análisis (COA)

Lot/Batch Number
70720200
39516300
24577100
53365800
13399600

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Haematologica, 106(11), 2960-2970 (2020-10-31)
The investigation of inherited disorders of erythropoiesis has elucidated many of the principles underlying the production of normal red blood cells and how this is perturbed in human disease. Congenital Dyserythropoietic Anaemia type 1 (CDA-I) is a rare form of
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