MAB3391
Anti-Collagen Type I (COL1A1) Antibody
CHEMICON®, mouse monoclonal, 5D8-G9
Sinónimos:
Anti-CAFYD, Anti-EDSARTH1, Anti-EDSC, Anti-OI1, Anti-OI2, Anti-OI3, Anti-OI4
About This Item
Productos recomendados
Nombre del producto
Anti-Collagen Type I Antibody, clone 5D8-G9, clone 5D8-G9, Chemicon®, from mouse
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
5D8-G9, monoclonal
reactividad de especies
pig, canine, sheep, human, bovine
no debe reaccionar con
guinea pig, ground squirrel, rat, kangaroo, horse, chicken, mouse
fabricante / nombre comercial
Chemicon®
técnicas
ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
isotipo
IgG1
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... COL1A1(1277)
Categorías relacionadas
Descripción general
Especificidad
Collagen Type I Antibody displays a high affinity for human, bovine and ovine Type I Collagens. There is no evidence for cross reactivity with Collagen Types III, V and VI or connective tissue protein. Antibody reacts only with native, non-denatured Collagen I
Aplicación
Immunohistochemistry:unfixed frozen sections:
Cut 6 μm thick sections from frozen samples using a freezing microtome. Air-dry throughly. Rehydrate with PBS. Blocking is usually not necessary for IF studies, if using DAB, pretreatment for peroxidase is suggested and blocking 2% BSA in PBS will reduce back ground.
Dilute Collagen Type I Antibody in PBS buffer, add to the sample(s) and incubate for 60 minutes. (1:100-1:500).
Wash sections for 10 minutes in PBS, twice. Add FITC conjugated anti-mouse antibody and incubate for 60 minutes.
Wash for 10 minutes in PBS, twice.
Mount sections in glycerol/water (9:1 v/v) containing 1 mM 1,4-phenylenediamine. (for FITC stability or use Chemicon catalog numbers 5096 or 5013.
NOTE:
Preliminary studies suggest that enhanced staining of certain tissues maybe obtained by pretreatment (previous to step 2) of sections with various enzymes (eg 0.1% pepsin in 0.1M HCl, 37°C for 5 minutes).
Flow Cytometry: 1:100
Immunoblotting:1:1000 using NATIVE, non-denatured, non-reduced western blots only. Ramshaw, et.al (1988) "Electrophoretic and electroblotting of native collagens." Anal. Biochem. 168:82-87. Antibody will not work in traditional, reduced western blots.
Briefly, PAGE gels must be prepared for running native collagens.
A 3% (w/v) total acrylamide separating gel, containing 3.2% (w/w) bis-acrylamide as a proportion to a monomer as the crosslinking agent, in 10mM calcium lactate, pH 6.8 is polymerized between vertical glass plates by the addition of 0.05-0.1% TEMED and 0.05-.1% ammonium persulfate (added from a 10% stock solution). The cathode buffer (negative) buffer is 50mM Tris adjusted to pH 6.6 with lactic acid; the anode (positive) buffer is 0.1M lactic acid pH 2.5 {Friis, SJ et al, 1985 J Biochem Biophys Methods 10:301-306.}.
Prior to sample loading the gel is run for at least 90 minutes at 100V. Collagen samples are dissolved at 1mg/mL in either 0.1M lactic acid or 0.1M acetic acid containing 10% sucrose, and 5-10μg per lane is loaded. Methyl green is added as required to assist loading. Electrophoresis is 70V for 5 hours, room temperature.
Blotting uses 0.1M lactic acid pH 2.5, and 20V for 16 hours at room temperature with the collagens migrating toward the cathode.
Cell Structure
ECM Proteins
Forma física
The immunoglobulin fraction has been purified by Protein A Chromatography and shown to be >95% pure by coomassie PAGE. The Collagen Type I Antibody is supplied 0.22 micron filtered.
Almacenamiento y estabilidad
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Nota de análisis
Human kidney lysate
Información legal
Cláusula de descargo de responsabilidad
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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