71194-M
0.5 M THP Solution
Reducing agent compatible with His•Bind chromatography
Sinónimos:
Tris(3-hydroxypropyl)phosphine
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About This Item
UNSPSC Code:
41106511
NACRES:
NA.77
Productos recomendados
Quality Level
form
solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
technique(s)
protein purification: suitable
application(s)
sample preparation
storage temp.
−20°C
General description
Reducing agent compatible with HIS-BIND® chromatography
THP [Tris(hydroxypropyl)phosphine; C9H21O3P] is a water-soluble, odorless, sulfhydryl reducing agent that is more stable and effective than dithiothreitol (DTT). Against oxidized glutathione, the reducing capacity of THP (0.232 meq/g) is greater than that of TCEP (0.182 meq/g). THP is also more resistant to air oxidation than DTT and is compatible with immobilized metal affinity chromatography with HIS-BIND® and Ni-NTA His•Bind resins at concentrations up to 1.0 mM. The common working concentration of THP for many applications is 15 mM. THP is provided as a ready-to-use 0.5 M solution in water.
Purity: >80% by NMR. CAS: 4706-17-6. MW: 208.2.
Purity: >80% by NMR. CAS: 4706-17-6. MW: 208.2.
Features and Benefits
Compatability with common methods for purifying His-tagged proteins - immobilized metal affinity chromatography with HIS-BIND® and Ni-NTA His•Bind resins at concentrations up to 1.0 mM
Stability: More stable and effective than traditional reducing agents like dithiothreitol (DTT)
Reducing Capacity: greater reducing capacity than TCEP against oxidized glutathione
Ready-to-use: THP is provided as a ready-to-use 0.5 M solution in water, making it easy to use in many applications with flexible usage
Stability: More stable and effective than traditional reducing agents like dithiothreitol (DTT)
Reducing Capacity: greater reducing capacity than TCEP against oxidized glutathione
Ready-to-use: THP is provided as a ready-to-use 0.5 M solution in water, making it easy to use in many applications with flexible usage
Warning
Toxicity: Corrosive (L)
Other Notes
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Legal Information
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Storage Class
8B - Non-combustible corrosive hazardous materials
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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