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Key Documents

H8629

Sigma-Aldrich

Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

lyophilized powder

Sinónimos:

HK/G6PD

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About This Item

MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

form

lyophilized powder

glucose-6-phosphate dehydrogenase activity

≥75 units/mg protein (biuret)

hexokinase activity

≥65 units/mg protein

purified by

crystallization

storage temp.

−20°C

General description

Research area: Cell signaling

Hexokinase and Glucose-6-phosphate Dehydrogenase are intracellular enzymes found distributed widely in the living cells.
Enzyme Commission numbers 2.7.1.1 + 1.1.1.49

Application

Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae) has been used to evaluate the adenylate kinase (Adk), activities of the mutant Adk’s. It has also been used for the determination of glucose and ATP.

Biochem/physiol Actions

Hexokinase initiates the glycolysis pathway by catalyzing the phosphorylation of glucose to Glucose-6-phosphate in the presence of ATP. Glucose-6-phosphate dehydrogenase plays a role in the subsequent oxidation of Glucose-6-phosphate in the presence of triphosphopyridine nucleotide (TPN). Hexokinase and Glucose-6-phosphate Dehydrogenase both serve as a tool in biochemical and analytical studies for determining glucose, fructose, mannose, ATP, and creatin-kinase activity.

Packaging

Package sizes are based on hexokinase units.

Components

Mixture of hexokinase and glucose-6-phosphate dehydrogenase

Unit Definition

Hexokinase Unit Definition: One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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