GE28-4009-89

His MultiTrap^™ High Performance

Cytiva 28-4009-89, pack of 4 plates

• Sinónimos: High Performance His Trap, His MultiTrap, MultiTrap for His
• Código UNSPSC: 12161501
• NACRES: NA.32

Propiedades

• Materiales: polypropylene and polyethylene
• envase: pack of 4 plates
• fabricante / nombre comercial: Cytiva 28-4009-89
• tamaño: 127.8 mm × 85.5 mm × 30.6 mm
• Matriz: highly cross-linked 6% agarose
• tam. prom. pieza: 34 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• Intervalo de trabajo: 3-12(Ni²⁺-stripped medium.)
• capacidad: ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

Descripción

Descripción general

His MultiTrap^™ FF and His MultiTrap^™ HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC).

Aplicación

His MultiTrap^™ FF and His MultiTrap^™ HP are prepacked 96-well filter plates for high-throughput screening of histidine-tagged* Proteins from clarified or unclarified lysates. Purification is performed by immobilized metal ion affinity chromatography (IMAC). The plates are prepacked with Ni Sepharose^™ 6 Fast FLow (His MultiTrap^™ FF) or Ni Sepharose^™ High Performance (His MultiTrap^™ HP). A protocol for His MultiTrap^™ is available in His Buffer Kit. The kit is available with ready-made buffer solutions, for increased convenience and for fast buffer preparation.

Loading unclarified samples directly to His MultiTrap^™ FF and His MultiTrap^™ HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target Proteins. His MultiTrap^™ FF and His MultiTrap^™ HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.

Características y beneficios

• Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* Proteins.
• Increased convenience and consistency with prepacked 96-well plates.
• No filtration needed - load unclarified sample directly, increase reproducibility, and save time.
• Use His MultiTrap^™ 96-well filter plates with robotic systems or manually with centrifugation or vacuum.
• Easy scale-up to HisTrap^™ FF, HisPrep^™ FF 16/10 or HisTrap^™ HP prepacked columns. High chemical stability and high binding capacity up to 1 mg of histidine-tagged Protein per well.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

HisPrep is a trademark of Cytiva

HisTrap is a trademark of Cytiva

MultiTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Información de seguridad

• Pictogramas: GHS09
• Palabra de señalización: Warning
• Frases de peligro: H226
• Consejos de prudencia: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de clase de almacenamiento: 3 - Flammable liquids