Saltar al contenido
Merck

11175033910

Roche

DIG DNA Labeling Kit

greener alternative

sufficient for 40 labeling reactions, kit of 1 (7 components), suitable for hybridization

Sinónimos:

dig, dna labeling kit, dig

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
41105500

usage

sufficient for 40 labeling reactions

Quality Level

packaging

kit of 1 (7 components)

manufacturer/tradename

Roche

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

hybridization: suitable

greener alternative category

storage temp.

−20°C

General description

DIG DNA Labeling Kit is a convenient kit for the labeling of DNA with Digoxigenin-deoxyuridine triphosphate (dUTP) using random oligonucleotides as primers. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Specificity

Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0) and/or by heating to 65 °C for 10 minutes.

Application

For Random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile. DIG-labeled DNA probes can be used for:
  • All types of filter hybridization according to our standard protocol given in the pack insert of the special hybridization solution DIG Easy Hyb.
  • Single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat.
  • In situ hybridizations

Packaging

1 kit containing 7 components.

Quality

Function tested in a Southern blot.

Specifications

Assay Time: Labeling: 1hour to O/N
Sensitivity and specificity: A single-copy human gene (tPA gene) is detected with a DIG-labeled probe in a Southern blot of 1μg digested human placenta DNA.

Principle

DIG-labeled DNA probes are generated according to the random-primed DNA labeling method which is based on the hybridization of random oligonucleotides to the denatured DNA template. The complementary DNA strand is synthesized by Klenow enzyme which uses the 3′-OH termini of the random oligonucleotides as primers and a mixture of deoxyribonucleotides containing DIG-11-dUTP, alkali-labile for elongation. This results in incorporation of digoxigenin into the newly synthesized DNA.

Note:
  • The use of the alkali-labile form of DIG-11-dUTP enables easier and more efficient stripping of blots for rehybridization experiments with a second DIG-labeled probe.
  • DNA probe, labeled with DIG-11-dUTP, alkali-labile must not be denatured using NaOH, but can be denatured by boiling in a waterbath.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Solo componentes del kit

Referencia del producto
Descripción

  • Unlabeled Control DNA 1 100 µg/ml

  • Unlabeled Control DNA 2 100 µg/ml

  • DNA Dilution Buffer

  • DIG-labeled Control DNA 5.2 µg/ml

  • Hexanucleotide Mix 10x concentrated

  • dNTP Labeling Mixture 10x concentrated

  • Klenow Enzyme, Labeling grade 2 U/µl

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Yanfang Du et al.
The New phytologist, 214(2), 721-733 (2017-01-04)
UNBRANCHED3 (UB3), a member of the SQUAMOSA promoter binding protein-like (SPL) gene family, regulates kernel row number by negatively modulating the size of the inflorescence meristem in maize. However, the regulatory pathway by which UB3 mediates branching remains unknown. We
Pedro Vallecillo-García et al.
Nature communications, 8(1), 1218-1218 (2017-11-01)
Fibro-adipogenic progenitors (FAPs) are an interstitial cell population in adult skeletal muscle that support muscle regeneration. During development, interstitial muscle connective tissue (MCT) cells support proper muscle patterning, however the underlying molecular mechanisms are not well understood and it remains
Laura Knuppertz et al.
Autophagy, 10(5), 822-834 (2014-03-04)
The filamentous ascomycete Podospora anserina is a well-established aging model in which a variety of different pathways, including those involved in the control of respiration, ROS generation and scavenging, DNA maintenance, proteostasis, mitochondrial dynamics, and programmed cell death have previously
Daniela Heinz et al.
Frontiers in cell and developmental biology, 9, 616520-616520 (2021-03-23)
Organismic aging is known to be controlled by genetic and environmental traits. Pathways involved in the control of cellular metabolism play a crucial role. Previously, we identified a role of PaCLPP, a mitochondrial matrix protease, in the control of the
Cristina Corral-Ramos et al.
Autophagy, 11(1), 131-144 (2015-01-07)
In the fungal pathogen Fusarium oxysporum, vegetative hyphal fusion triggers nuclear mitotic division in the invading hypha followed by migration of a nucleus into the receptor hypha and degradation of the resident nucleus. Here we examined the role of autophagy

Artículos

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico