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504971

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Discovery® C18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

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About This Item

Código UNSPSC:
41115700
eCl@ss:
32110501
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nombre del producto

Columna para HPLC Discovery® C18, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

Agency

suitable for USP L1

Nivel de calidad

100

Características

endcapped

fabricante / nombre comercial

Discovery®

Extensión del etiquetado

12% Carbon loading

Parámetros

≤70 °C temp. range
400 bar pressure (5801 psi)

técnicas

HPLC: suitable

L × D.I.

25 cm × 4.6 mm

superficie

300 m2/g

cobertura de la superficie

3 μmol/m2

impurezas

<10 ppm metals

Matriz

silica gel, high purity, spherical particle platform

grupo activo de la matriz

C18 (octadecyl) phase

tamaño de partícula

5 μm

tamaño de poro

180 Å

Intervalo de pH operativo

2-8

aplicaciones

food and beverages

técnica de separación

reversed phase

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Descripción general

Utilice Discovery® C18 para cualquier método que especifique una C18. La forma del pico, la reproducibilidad y la estabilidad excepcionales hacen de ella la columna de elección para todos los métodos C18, desde los más exigentes hasta los rutinarios.

Características y beneficios

  • Excelente reproducibilidad
  • Excepcional forma del pico para analitos básicos y ácidos
  • Separaciones LC-MS estables con poco sangrado
  • Separación de péptidos y proteínas pequeñas
  • Menor hidrofobicidad que muchas columnas C 18 comparables, proporcionando análisis más rápidos

Productos recomendados

Descubra los reactivos LiChropur ideales para análisis mediante HPLC o LC-MS

Información legal

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Aplicación

Referencia del producto
Descripción
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Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
294410-06
294410-05
294410-04
294410-03
294410-02

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T Nagano et al.
Journal of dental research, 88(9), 823-828 (2009-09-22)
Mmp-20 and Klk4 are the two key enamel proteases. Can both enzymes process amelogenin to generate the major cleavage products that accumulate during the secretory stage of amelogenesis? We isolated Mmp-20 and Klk4 from developing pig teeth and used them
T R Shantha Kumar et al.
Journal of pharmaceutical and biomedical analysis, 38(1), 173-179 (2005-05-24)
A simple, precise and accurate isocratic reverse-phase liquid chromatography method with programmed wavelength detection has been validated to quantify DRF-4367 and Phenol red, simultaneously for application in rat in situ single pass intestinal perfusion study to assess intestinal permeability of
Ramisetti Nageswara Rao et al.
Journal of separation science, 32(9), 1312-1322 (2009-04-29)
Modafinil, adrafinil and their related substances were synthesized and analyzed by RP-LC with ESI-MS/MS. The ionization mode, polarity, cone voltage, and chromatographic conditions were evaluated. The optimum LC-MS conditions to obtain fragment ions indispensable for identification of the structures were
Himal Paudel Chhetri et al.
Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 22(5), 483-487 (2014-12-05)
This study presents the optimization of a simple HPLC-UV method for the determination of metformin in human plasma. Ion pair separation followed by UV detection was performed on deproteinized human plasma samples. The separation was carried out on a Discovery
Abbas Khan et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(24), 2423-2429 (2011-07-26)
A novel isocratic reversed-phase high performance liquid-chromatography/ultraviolet detection method for simultaneous determination of cefdinir and cefixime in human plasma was developed and validated after optimization of various chromatographic conditions and other experimental parameters. Sample preparation based on a simple extraction
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Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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