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Y2377

Sigma-Aldrich

2x YT medium

Powder microbial growth medium

Sinónimos:

2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media

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About This Item

Código UNSPSC:
41106225
NACRES:
NA.85

grado

for molecular biology

esterilidad

non-sterile

Formulario

powder

técnicas

microbiological culture: suitable

pH

6.8-7.2(3.1% solution)

aplicaciones

food and beverages
microbiology

temp. de almacenamiento

room temp

idoneidad

nonselective for Escherichia coli
nonselective for coliforms

Descripción general

2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Aplicación

2x YT medium has been used for culturing E. coli BL21 for cloning , E. coli SS320 cells for scaffold synthesis , and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens and E. coli DH10B.

Características y beneficios

2xYT powder provides:
  • Standard formulation
  • An inexpensive alternative to liquid

Componentes

16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl

Nota de preparación

1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.

Reconstitución

Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Producto relacionado

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000387739
0000387739
SLCR2623
0000344677
0000343368

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Protocolos

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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