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T8665

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, liquid

Sinónimos:

TMB

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About This Item

Código UNSPSC:
12352204
NACRES:
NA.83

Nombre del producto

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System, peroxidase substrate

Nivel de calidad

300

Formulario

liquid

condiciones de almacenamiento

protect from light

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Categorías relacionadas

Aplicación

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Precaución

This substrate is very light-sensitive and should be protected from direct sunlight or UV sources.

Forma física

Ready-to-use.

Otras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 3 - STOT SE 1

Órganos de actuación

Eyes,Central nervous system

Clase de riesgo para el agua (WGK)

WGK 2

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Certificados de análisis (COA)

Lot/Batch Number
SLCP9930
SLCP9930
SLCP1175
SLCP1175
0000401097

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S Madersbacher et al.
Methods (San Diego, Calif.), 21(1), 41-50 (2000-04-15)
As a glycoprotein hormone, human chorionic gonadotropin (hCG) is not a single molecular entity. This term comprises not only the bioactive heterodimer hCG but also an array of molecular protein backbone and glycosylation variants, such as its free beta (hCGbeta)
Paweł Kozielewicz et al.
Molecular pharmacology, 97(1), 23-34 (2019-11-11)
Smoothened (SMO) is a GPCR that mediates hedgehog signaling. Hedgehog binds the transmembrane protein Patched, which in turn regulates SMO activation. Overactive SMO signaling is oncogenic and is therefore a clinically established drug target. Here we establish a nanoluciferase bioluminescence
Sui-Yuan Chang et al.
Scientific reports, 11(1), 8692-8692 (2021-04-24)
A metal nanoparticle composite, namely TPNT1, which contains Au-NP (1 ppm), Ag-NP (5 ppm), ZnO-NP (60 ppm) and ClO2 (42.5 ppm) in aqueous solution was prepared and characterized by spectroscopy, transmission electron microscopy, dynamic light scattering analysis and potentiometric titration. Based on the in
Tomasz Brudek et al.
Molecular neurodegeneration, 12(1), 44-44 (2017-06-09)
Parkinson's' disease (PD) and Multiple System Atrophy (MSA) are progressive brain disorders characterized by intracellular accumulations of α-synuclein and nerve cell loss in specific brain areas. This loss causes problems with movement, balance and/or autonomic functions. Naturally occurring autoantibodies (NAbs)
Janine Wesslowski et al.
The Journal of biological chemistry, 295(26), 8759-8774 (2020-05-10)
The Wingless/Int1 (Wnt) signaling system plays multiple, essential roles in embryonic development, tissue homeostasis, and human diseases. Although many of the underlying signaling mechanisms are becoming clearer, the binding mode, kinetics, and selectivity of 19 mammalian WNTs to their receptors
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