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SRP6489

Sigma-Aldrich

Carbonic Anhydrase 4/CA4 human

recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)

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About This Item

Código UNSPSC:
12352204
NACRES:
NA.77

origen biológico

human

recombinante

expressed in HEK 293 cells

etiqueta

6-His tagged (C-terminus)

Ensayo

≥92% (SDS-PAGE)

Formulario

lyophilized

mol peso

calculated mol wt 31 kDa
observed mol wt 33 kDa by SDS-PAGE (reducing) (Ala 19 is the predicted N-terminal .)

envase

pkg of 10 μg

impurezas

<1 EU/μg endotoxin (LAL test)

Nº de acceso UniProt

P22748

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

human ... CA4(762)

Descripción general

Carbonic anhydrase 4 (CA4) is a 35kDa protein, encoded by the gene mapped to human chromosome 17q23.1-23.2. It is expressed on extracellular surfaces of certain endothelial and epithelial cells. CA4 is one among the 12 isozymes of carbonic anhydrase.
Carbonic anhydrase 4 (CA4) is also known as Carbonate dehydratase IV, Carbonic anhydrase IV, which belongs to the α-carbonic anhydrase family. CA4 may stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. The enzyme regulation is activated by histamine, L-adrenaline, D-phenylalanine, L- and D-histidine. CA4 can interact with SLC4A4.

Acciones bioquímicas o fisiológicas

Carbonic anhydrase 4 (CA4) plays a vital role in CO2 hydration activity. It also catalyzes the dehydration of HCO3. In addition, it also facilitates CO2 and HCO3 transport in various vital tissues like kidney and lung Polymorphism in the gene has been associated with the development of sporadic retinitis pigmentosa (RP).

Forma física

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number

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Li-Li Huang et al.
Journal of clinical and translational hepatology, 11(3), 605-613 (2023-03-28)
Hepatocellular carcinoma (HCC) is one of the most common types of cancer, often resulting in death. Augmenter of liver regeneration (ALR), a widely expressed multifunctional protein, has roles in liver disease. In our previous study, we reported that ALR knockdown
Familial microduplication of 17q23.1?q23.2 involving TBX4 is associated with congenital clubfoot and reduced penetrance in females.
Peterson J F, et al.
American Journal of Medical Genetics. Part A, 164(2), 364-369 (2014)
Screening for the Carbonic Anhydrase IV Gene Mutations in Chinese Retinitis Pigmentosa Patients
Tian Y, et al.
Current Eye Research, 35(5), 440-444 (2010)
Membrane associated carbonic anhydrase IV (CA IV): a personal and historical perspective.
Waheed A, et al.
Sub-Cellular Biochemistry, 157-179 (2014)
Rupak Datta et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(9), 3437-3442 (2009-02-13)
Missense mutations in the carbonic anhydrase IV (CA IV) gene have been identified in patients with an autosomal dominant form of retinitis pigmentosa (RP17). We used two transient expression systems to investigate the molecular mechanism by which the newly identified

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