SRP3171

TGF-beta 3-E. coli human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Sinónimos: Transforming Growth Factor-beta3
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in E. coli
• Ensayo: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Formulario: lyophilized
• potencia: ≤0.05 ng/mL ED₅₀
• mol peso: 25.0 kDa
• envase: pkg of 10 μg
• técnicas: cell culture | mammalian: suitable
• impurezas: <0.1 EU/μg endotoxin, tested
• color: white
• Nº de acceso UniProt: P10600
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... TGFB3(7043)

Descripción

Descripción general

The gene TGF-β3 (transforming growth factor β 3) is mapped to human chromosome 14q24.3. The three mammalian isoforms of TGF-β, TGF-β1, β2, β3 elicit similar biological responses. TGF-β proteins are made as precursor molecules which are cut to generate a mature carboxy-terminal region of 110-140 amino acids. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. Recombinant TGF-β3 is a 25.0kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

Aplicación

TGF-β3 (transforming growth factor β 3) human has been used in the media for the chondrogenic differentiation of the stromal cells. It has been used in the culture medium to allow attachment of primary human dermal fibroblasts to the scaffolds.

Acciones bioquímicas o fisiológicas

The three mammalian isoforms of TGF-β (transforming growth factor β), TGF-β1, β2, β3, are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. TGF-β3 is the predominant TGF-β in the testis and controls the blood-testis barrier activity. Polymorphisms in this gene might be associated with male infertility. Heterozygous mutation in the gene at position Arg300 might be associated with increased TGF-β signaling, leading to a disorder with phenotypic overlap with Marfan syndrome and Loeys-Dietz syndrome. TGF-β3 is involved in the mesenchymal stem cells (MSCs)-mediated inhibition of fibroblast proliferation and decreases skin fibrosis.

Secuencia

ALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS

Forma física

Lyophilized from 0.1% TFA

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 °C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 °C to -80 °C.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable