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QPBCA

Sigma-Aldrich

QuantiPro™ BCA Assay Kit

for 0.5-30 μg/ml protein

Sinónimos:

BCA Assay, Protein determination by BCA, bicinchoninic acid reagent

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

Nivel de calidad

200

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Categorías relacionadas

Aplicación

In addition to protein determination and quantification in solution, the QuantiPro™ BCA Assay Kit has other applications, including determination of protein covalently bound to agarose supports and protein adsorbed to multiwell plates. It has also been applied to the determination of functional groups such as sulfhydryl, N-hydroxysuccinimido carboxylate, aldehyde and hydrazide on a variety of solid supports.
The kit can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5 to 30 μg/mL in tube assays and 1 to 20 μg/mL in 96- or 384-well plate assays.

Características y beneficios

  • Accurate across a broad range of protein concentrations
  • High sensitivity; linear response form 0.5 to 30 μg/ml of protein
  • Stable color complex
  • Reduced susceptibility to detergents

Principio

Based on the alkaline reduction of Cu2+ to Cu1+ by proteins, and the formation of a bicinchoninic acid:Cu1+ complex having an absorbance maximum at 562 nm.

Almacenamiento y estabilidad

Store Reagents QA, QB, and QC at room temperature. Reagents QA and QB can be mixed together without Reagent QC (4% Copper(II) Sulfate Pentahydrate Solution 12 mL) and stored in a closed container at room temperature for a week. If this kit is received or stored cold, a precipitate may form in Reagent QA or Reagent QB. To dissolve the precipitate, warm the solution slowly to room temperature while mixing. The solutions are now suitable for use.

Información legal

QuantiPro is a trademark of Sigma-Aldrich Co. LLC

Solo componentes del kit

Referencia del producto
Descripción
  • Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • P0914Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1SDS

Aplicación

Referencia del producto
Descripción
Precios

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Timestrip Plus™ 8 °C

Pictogramas

CorrosionEnvironment
GHS05,GHS09

Palabra de señalización

Danger

Frases de peligro

H290,H315,H318,H410

Clasificaciones de peligro

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

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Certificados de análisis (COA)

Lot/Batch Number
0000395428
0000364131
0000312919
0000331185
0000364131

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