MCF7 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012803, human breast (adenocarcinoma), Epithelial-like

• Sinónimos: IBMF-7 Cells, MCF7 Cells, Michigan Cancer Foundation-7 Cells, ssMCF-7 Cells
• Código UNSPSC: 41106514
• eCl@ss: 32190102

Propiedades

• origen biológico: human breast (adenocarcinoma)
• envase: tube of 5 μg 86012803-DNA-5UG; pkg of vial of cells 86012803-1VL
• modo de crecimiento: Adherent
• cariotipo: 2n = 46, hypertriploid to hypotetraploid
• morfología: Epithelial-like
• productos: Not specified
• receptores: Reported to express the oestrogen and progesterone receptor
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian breast adenocarcinoma

Descripción línea celular

Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.

Aplicación

Tumourigenicity and virus studies: Type B and C viruses

Perfil del ADN

STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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