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P5368

Disolución salina tamponada con fosfato

powder, pH 7.4, BioPerformance Certified, for molecular biology

Sinónimos:

PBS

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A ustedes/SKUDisponibilidadPrecio
10 pkg

Disponible para enviar HOYdesdePUDAHUEL

$77.100
5 x 10 pkg
Comprobar disponibilidad del carrito
$408.000

Acerca de este artículo

UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25
MDL number:

$77.100


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Nombre del producto

Disolución salina tamponada con fosfato, BioPerformance Certified, pH 7.4

Quality Segment

grade

BioPerformance Certified, Molecular Biology

form

powder

storage condition

(Keep container tightly closed in a dry and well-ventilated place.)

technique(s)

immunofluorescence: suitable

impurities

BioBurden, tested, endotoxin, tested

color

white

pH

7.4

solubility

water: 9.07-10.0 g/L, clear, colorless

cation traces

Fe: ≤10 ppm, heavy metals (as Pb): ≤5 ppm

suitability

suitable for SDS-PAGE, suitable for molecular biology

application(s)

diagnostic assay manufacturing
histology
sample preparation

foreign activity

DNase, RNase, Protease, none detected

SMILES string

OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]

InChI

InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5

InChI key

LOKCTEFSRHRXRJ-UHFFFAOYSA-I

Application


  • Extracción rápida y a coste cero del ADN de insectos de cuerpo blando para aplicaciones de PCR habituales: en este estudio se utilizó disolución salina tamponada con fosfato (PBS) para desarrollar un método rentable y eficiente para la extracción del ADN de los insectos, facilitando las aplicaciones habituales de PCR en biología molecular (Jangra & Ghosh, 2022).

  • Administración inyectable mediante hidrogel de amnios de células madre adiposas para el tratamiento de la artrosis: se utilizó PBS para preparar y administrar células madre derivadas del tejido adiposo en un hidrogel inyectable, lo que demostró potencial para tratamientos innovadores de la artrosis (Bhattacharjee et al., 2022).

  • Detección rápida y sensible de la infección por SARS-CoV-2 mediante análisis cuantitativo de enriquecimiento peptídico con LC-MS: en esta investigación se empleó PBS para la preparación de muestras en un nuevo método de LC-MS para detectar el SARS-CoV-2, proporcionando una herramienta de diagnóstico rápida y sensible (Hober et al., 2021).

Packaging

Bolsas de aluminio

Preparation Note

El contenido de una bolsa, cuando se disuelve en un litro de agua destilada o desionizada, producirá disolución salina tamponada con fosfato 0,01 M (NaCl 0,138 M; KCl - 0,0027 M); pH 7,4, a 25 °C.

Other Notes

Si desea más información sobre nuestra gama de productos bioquímicos, cumplimente este formulario.

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1 of 1

Este artículo
P3744P3813P38135
description

BioPerformance Certified, pH 7.4

description

pH 7.6 (25 °C)

description

powder, pH 7.4, for preparing 1 L solutions

description

powder, pH 7.4, for preparing 5 L solutions

grade

BioPerformance Certified, Molecular Biology

grade

-

grade

-

grade

-

technique(s)

immunofluorescence: suitable

technique(s)

-

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

application(s)

diagnostic assay manufacturing
histology
sample preparation

application(s)

diagnostic assay manufacturing

application(s)

advanced drug delivery
diagnostic assay manufacturing
life science and biopharma
sample preparation

application(s)

diagnostic assay manufacturing

form

powder

form

powder

form

powder

form

powder

pH

7.4

pH

7.6 (25 °C)

pH

7.4

pH

7.4


Still not finding the right product?


Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–7 of 7 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the shelf-life of product P5368, phosphate buffered saline (PBS)?

    1 answer
    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. If reconstituted with sterile water, will Product P5368, Phosphate buffered saline (PBS), be sterile?

    1 answer
    1. No.  To make this PBS sterile, the solution should be either sterile-filtered or autoclaved.

      Helpful?

  6. What volume of Product P5368, Phosphate buffered saline (PBS), does each pack make?

    1 answer
    1. When the contents of a pouch is dissolved in 1 Liter of water, it makes 1X PBS, pH 7.4 at 25°C.

      Helpful?

  7. Does Product P5368, Phosphate buffered saline (PBS), contain calcium and magnesium?

    1 answer
    1. No.  The complete formulation is shown in the product listing.  When reconstituted, the resulting solution is 10 mM phosphate buffer, with 138 mM sodium chloride and 2.7 mM potassium chloride.

      Helpful?

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