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Merck

P2545

Millipore

Proteína A – Agarosa

saline suspension

Sinónimos:

Protein A resin

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About This Item

Número MDL:
Código UNSPSC:
41106500
NACRES:
NA.56

origen biológico

Staphylococcus aureus

Nivel de calidad

Formulario

saline suspension

Extensión del etiquetado

~2 mg per mL

activación de la matriz

cyanogen bromide

unión a la matriz

amino

espaciador de matriz

1 atom

capacidad

≥15 mg/mL binding capacity (human IgG)

temp. de almacenamiento

2-8°C

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Descripción general

Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.

Aplicación

Protein A−Agarose has been used in the immunoprecipitation
  • of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
  • of prolyl hydroxylase domain 2 N27 cells
  • of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Acciones bioquímicas o fisiológicas

Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications.

Forma física

Suspension in 0.5 M NaCl containing preservative

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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PTEN-Dependent Stabilization of MTSS1 Inhibits Metastatic Phenotype in Pancreatic Ductal Adenocarcinoma
Zeleniak AE, et al.
Neoplasia, 20(1), 12-24 (2018)
D J McCool et al.
The Biochemical journal, 302 ( Pt 1), 111-118 (1994-08-15)
Pulse-chase labelling experiments were performed using the mucin-producing colonic carcinoma cell line LS180. Cells were pulsed with [3H]threonine or [3H]glucosamine and chased in complete media without radiolabel for various lengths of time. From cell and media extracts obtained at each
Hsp90 Co-chaperone p23 contributes to dopaminergic mitochondrial stress via stabilization of PHD2: Implications for Parkinson?s disease
Rane A, et al.
Neurotoxicology, 65, 166-173 (2018)
Madhu Chhanda Mohanty et al.
Developmental and comparative immunology, 26(4), 385-391 (2002-03-13)
Meriones unguiculatus commonly known as gerbils are widely used as animal models for a variety of parasitic infections such as Brugia malayi, Entamoeba histolytica, Giardia duodenalis, Toxoplasma gondi, Helicobacter pylori, Strongyloides stercoralis and Echinococcus multilocularis. Groups of BALB/c mice, gerbils
D J McCool et al.
The Biochemical journal, 341 ( Pt 3), 593-600 (1999-07-27)
Molecular chaperones are presumed to associate with large secretory mucin glycoproteins during their synthesis in the endoplasmic reticulum (ER), but have not been identified to date. We decided to look for possible involvement of the chaperones calreticulin (CRT) and calnexin

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