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Merck
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P9424

Millipore

Proteína A – Agarosa

aqueous ethanol suspension

Sinónimos:

Protein A–Agarose

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About This Item

MDL number:
MFCD00165563
UNSPSC Code:
41106500
NACRES:
NA.56

biological source

Staphylococcus aureus

Quality Level

100

form

aqueous ethanol suspension

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

extent of labeling

~6 mg per mL

technique(s)

affinity chromatography: suitable

matrix

Sepharose 4B Fast Flow

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥30 mg/mL binding capacity (human IgG)

storage temp.

2-8°C

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Categorías relacionadas

General description

Protein A-Sepharose™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.

Application

Protein A-Sepharose is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.
Protein A-Sepharose™ Fast Flow from Staphylococcus aureus has been used:
  • in co-immunoprecipitation assay
  • in immunodepletion
  • to purify IgG from human serum and plasma

Physical form

Suspension in 20% ethanol

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

wgk_germany

WGK 3

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János Roszik et al.
European journal of immunology, 41(5), 1288-1297 (2011-04-07)
T-cell receptors (TCRs) can be genetically modified to improve gene-engineered T-cell responses, a strategy considered critical for the success of clinical TCR gene therapy to treat cancers. TCR:ζ, which is a heterodimer of TCRα and β chains each coupled to
Yuhua Zhang et al.
Plant physiology, 149(4), 1860-1871 (2009-02-06)
Trehalose-6-phosphate (T6P) is a proposed signaling molecule in plants, yet how it signals was not clear. Here, we provide evidence that T6P functions as an inhibitor of SNF1-related protein kinase1 (SnRK1; AKIN10/AKIN11) of the SNF1-related group of protein kinases. T6P
Joyce A Benjamins et al.
Neurology(R) neuroimmunology & neuroinflammation, 6(3), e550-e550 (2019-05-03)
To identify whether factors toxic to oligodendrocytes (OLs), released by B cells from patients with MS, are found in extracellular microvesicles enriched in exosomes. Conditioned medium (Sup) was obtained from cultures of blood B cells of patients with MS and
H Sternlicht et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(20), 9422-9426 (1993-10-15)
A role in folding newly translated cytoskeletal proteins in the cytosol of eukaryotes has been proposed for t-complex polypeptide 1 (TCP1). In this study, we investigated tubulin and actin biogenesis in Chinese hamster ovary (CHO) cells. When extracts of pulse-labeled
Lydia Alvarez-Erviti et al.
Neurobiology of disease, 42(3), 360-367 (2011-02-10)
Alpha-synuclein aggregation plays a central role in Parkinson's disease pathology. Direct transmission of alpha-synuclein from pathologically affected to healthy unaffected neurons may be important in the anatomical spread of the disease through the nervous system. We have demonstrated that exosomes
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