M8284
Maltose Phosphorylase from Enterococcus sp.
recombinant, expressed in E. coli, lyophilized powder
Sinónimos:
Maltose:orthophosphate 1-β-D-Glucosyltransferase
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About This Item
Productos recomendados
recombinante
expressed in E. coli
Nivel de calidad
Formulario
lyophilized powder
actividad específica
≥9 units/mg solid
mol peso
90 kDa by SDS-PAGE
temp. de almacenamiento
−20°C
cadena SMILES
OC(=O)CCCCCCCCCCCCC
InChI
1S/C14H28O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14(15)16/h2-13H2,1H3,(H,15,16)
Clave InChI
TUNFSRHWOTWDNC-UHFFFAOYSA-N
Categorías relacionadas
Descripción general
Maltose phosphorylase (MP) is a dimeric enzyme. This enzyme is classified under family 65 of the glycoside hydrolases. It is a member of the disaccharide phosphorylase family.
Aplicación
Maltose Phosphorylase from Enterococcus sp. has been used as a component in coupled assay and ATPase activity assay to study its effects on ATPase activity, on the 90 kDa heat shock proteins (Hsp90) ATPase reaction and on background absorbance on the production of resorufin.
Maltose phosphorylase from Enterococcus has been used in a study to describe a new pathway for maltose utilization in lactic acid bacteria. It has also been used in a study to describe the transfer of glucosyl moiety of maltose to acceptors with alcoholic OH groups.
Acciones bioquímicas o fisiológicas
Enzymatically converts maltose to D-Glucose.
Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes maltose and inorganic phosphate into β-D-glucose-1-phosphate and glucose.
Maltose phosphorylase not only transfers glucosyl moieties from maltose to other sugars but has been shown to also use phenolic compounds such as salicyl alcohol as acceptors.
Definición de unidad
One unit will produce 1.0 μmole of D-Glucose from maltose per minute at pH 7.0 at 30°C.
Otras notas
Contains lactose.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Los clientes también vieron
M Tangney et al.
FEMS microbiology letters, 76(1-2), 191-196 (1992-10-01)
Extracts prepared from cultures of Bacillus subtilis, grown on maltose as the sole carbon source, lacked maltose phosphotransferase system activity. There was, however, evidence for a maltose phosphorylase activity, and such extracts also possessed both glucokinase and glucose phosphotransferase system
Y Le Breton et al.
Journal of applied microbiology, 98(4), 806-813 (2005-03-09)
The aim of this research was to characterize the metabolic pathway for maltose utilization in Enterococcus faecalis. Screening a library of Enterococcus faecalis insertional mutants allowed the isolation of mutants affected in maltose utilization. Genetic analysis of the insertion loci
U Nilsson et al.
Microbiology (Reading, England), 147(Pt 6), 1565-1573 (2001-06-08)
Maltose phosphorylase (MP) from Lactococcus lactis was purified and the corresponding gene was cloned and expressed in Escherichia coli. The isoelectric point of the pure enzyme was determined to be 7.0. According to zymogram analysis and SDS-PAGE, the native MP
Hiroyuki Nakai et al.
Carbohydrate research, 345(8), 1061-1064 (2010-04-16)
Lactobacillus acidophilus NCFM maltose phosphorylase (LaMalP) of glycoside hydrolase family 65 catalysed enzymatic synthesis of alpha-(1-->4)-glucosidic disaccharides from maltose and five monosaccharides in a coupled phosphorolysis/reverse phosphorolysis one-pot reaction. Thus phosphorolysis of maltose to beta-glucose 1-phosphate circumvented addition of costly
Ulrika Andersson et al.
BMC microbiology, 2, 28-28 (2002-09-26)
Maltose metabolism is initiated by an ATP-dependent permease system in Lactococcus lactis. The subsequent degradation of intracellular maltose is performed by the concerted action of Pi-dependent maltose phosphorylase and beta-phosphoglucomutase. In some Gram-positive bacteria, maltose metabolism is regulated by a
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