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H9037

Sigma-Aldrich

Anti-HA monoclonal

~1 mg/mL, clone HA-7, purified from hybridoma cell culture

Sinónimos:

Anti-HA, Hemaglutinina anti-gripal

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About This Item

MDL number:
MFCD00803873
UNSPSC Code:
12352203
NACRES:
NA.56

biological source

mouse

conjugate

TRITC conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HA-7, monoclonal

form

buffered aqueous solution

storage condition

protect from light

concentration

~1 mg/mL

technique(s)

direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

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General description

Monoclonal Anti-HA is derived from the HA-7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Monoclonal anti-HA recognizes the HA tag sequence on HA tagged fusion proteins by immunocytochemistry. The antibody reacts specifically with N- and C-terminal HA tagged fusion proteins.

Specificity

El anticuerpo reconoce tanto las formas nativas como las desnaturalizadas-reducidas de las proteínas etiquetadas con HA y es reactivo con las proteínas de fusión etiquetadas con HA en N-terminal o C-terminal expresadas en E. coli o en células de mamífero.

Immunogen

péptido sintético correspondiente a un fragmento de la hemaglutinina (HA) del virus de la gripe humana, conocida como etiqueta-HA, conjugado con KLH

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Monoclonal Anti-HA-TRITC antibody was used to test if DOT/ICM effector SDHA is necessary for virulence of Legionella pneumophila in Galleria mellonella and A/J mice.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin 15 mM sodium azide as a preservative.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Yasuhiro Yamazaki et al.
Science immunology, 5(44) (2020-03-01)
We investigated the molecular and cellular basis of severe combined immunodeficiency (SCID) in six patients with otofaciocervical syndrome type 2 who failed to attain T cell reconstitution after allogeneic hematopoietic stem cell transplantation, despite successful engraftment in three of them.
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Current opinion in biotechnology, 4(5), 520-525 (1993-10-01)
Recent advances in protein expression in E. coli have focused primarily on the enhancement of protein quality. Problems in mRNA translation such as inefficient initiation, mistranslation, frame-shifting and frame-hopping can often be addressed by altering heterologous gene-coding sequences. Fusion technology
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Infection and immunity, 81(7), 2598-2605 (2013-05-08)
Legionella pneumophila is an intracellular bacterium that resides within amoebae and macrophages in a specialized compartment termed the Legionella-containing vacuole (LCV). As well as providing an intracellular niche for replication, the LCV helps to prevent the release of bacterial components
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Legionella pneumophila is a bacterial pathogen that thrives in alveolar macrophages, causing a severe pneumonia. The virulence of L. pneumophila depends on its Dot/Icm type IV secretion system (T4SS), which delivers more than 300 effector proteins into the host, where
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