G9284

Guanidine hydrochloride solution

Colorless liquid, 7.8 - 8.3 M, pH- 4.5 - 5.5

• Sinónimos: Aminoformamidine hydrochloride, Guanidinium chloride, Guanidium chloride
• Fórmula empírica (notación de Hill): CH₅N₃ · HCl
• Número de CAS: 50-01-1
• Peso molecular: 95.53
• Beilstein: 3591990
• Número MDL: MFCD00013026
• Código UNSPSC: 12352107
• ID de la sustancia en PubChem: 24895369
• NACRES: NA.55

Propiedades

• Nivel de calidad: 200
• Formulario: liquid
• densidad: 1.18 g/mL at 25 °C (lit.)
• cadena SMILES: Cl.NC(N)=N
• InChI: 1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H
• Clave InChI: PJJJBBJSCAKJQF-UHFFFAOYSA-N
• Información sobre el gen: human ... KCNA1(3736) , KCNA10(3744) , KCNA2(3737) , KCNA3(3738) , KCNA4(3739) , KCNA5(3741) , KCNA6(3742) , KCNA7(3743) , KCNB1(3745) , KCNB2(9312) , KCNC1(3746) , KCNC2(3747) , KCNC3(3748) , KCNC4(3749) , KCND1(3750) , KCND2(3751) , KCND3(3752) , KCNF1(3754) , KCNG1(3755) , KCNG2(26251) , KCNG3(170850) , KCNG4(93107) , KCNH1(3756) , KCNH2(3757) , KCNH3(23416) , KCNH4(23415) , KCNH5(27133) , KCNH6(81033) , KCNH7(90134) , KCNH8(131096) , KCNQ1(3784) , KCNQ2(3785) , KCNQ3(3786) , KCNQ4(9132) , KCNQ5(56479) , KCNS1(3787) , KCNS2(3788) , KCNS3(3790) , KCNV1(27012) , KCNV2(169522)

Descripción

Aplicación

Guanidine hydrochloride solution has been used:

• for protein denaturation
• to homogenize hypothalamus, thalamus, prefrontal cortex, and brainstem samples for total β-amyloid (Aβ) extraction and Aβ42 quantification
• as a chaotropic denaturant to treat human immunodeficiency virus-1 (HIV-1) samples to study its effects on HIV-1 sensitivity and to assay protein stability

Acciones bioquímicas o fisiológicas

Guanidine‐HCl (Gnd-HCl), an efficient chaotropic agent, is broadly used in RNA isolation, globular protein denaturation, and protein refolding studies as a denaturant. It is an appropriate sample buffer for the quick and effective elution of affinity-purified protein complexes and the subsequent protein digestion step. GnHCl can be used to extract proteins in extracellular matrix (ECM)‐rich tissues including bone, cartilage, ligament, and tendon. Gnd-HCl is not used in proteomics experiments due to its interference with trypsin activity at low molar concentrations and tendency to precipitate when combined with sodium dodecyl sulfate (SDS). It is appropriate for performing rapid and single-step affinity-purification mass spectrometry (AP-MS) experiments.

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H302 + H332,H315,H319
• Consejos de prudencia: P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Clasificaciones de peligro: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter