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Merck

D7304

Sigma-Aldrich

3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride

Sinónimos:

Chromogenic substrate, DAB substrate solution

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1 SET
$456.000

$456.000


Fecha estimada de envío19 de mayo de 2025


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1 SET
$456.000

About This Item

Código UNSPSC:
12352203
NACRES:
NA.46

$456.000


Fecha estimada de envío19 de mayo de 2025


Solicitar un pedido a granel

Formulario

liquid

Nivel de calidad

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Descripción general

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme and is useful as a staining agent in immunohistological[1] and immunoblotting techniques. The end product is resistant to alcohol therefore, a variety of counterstains and mounting media can be used with the DAB Liquid Substrate System. The DAB Liquid Substrate System provides all the chromogen and buffer/peroxide solutions needed to produce a fast and convenient DAB substrate solution..

Aplicación

3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride has been used as a substrate:
  • in the immunoblot assay carried out to identify the changes in heat shock proteins (Hsps) expressed in the liver[2]
  • for visualization of sections after immunostaining[3]
  • in immunohistochemistry of mice knee joint sections[4]
  • in the immunolabeling of kisspeptin neurons in the preoptic area of camel[5]
It produces a brown precipitate with peroxidase for use in immunohistological and immunoblotting staining procedures. Not recommended for ELISA (microwell) procedures.

Precaución

Stable at least one year at 2-8 °C.

Cantidad

Each DAB Liquid Substrate System will provide 250 mL of DAB substrate solution.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Solo componentes del kit

Referencia del producto
Descripción

  • 10X DAB Liquid Chromogen 25 mL

  • ready-to-use buffer/peroxide solution 250 mL

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Carc. 1B - Flam. Liq. 2 - STOT SE 1

Órganos de actuación

Eyes,Central nervous system

Código de clase de almacenamiento

3 - Flammable liquids

Clase de riesgo para el agua (WGK)

WGK 3


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Jennifer Walshe et al.
Developmental biology, 264(2), 522-536 (2003-12-04)
Characterisation of human craniofacial syndromes and studies in transgenic mice have demonstrated the requirement for Fgf signalling during morphogenesis of membrane bone of the cranium. Here, we report that Fgf activity is also required for development of the oro-pharyngeal skeleton
Adriana Martins Anciães et al.
Clinics (Sao Paulo, Brazil), 66(10), 1797-1803 (2011-10-21)
To verify the accordance of functional and morphometric parameters during the development of emphysema. BALB/c mice received a nasal drop of either papain or saline solution and were studied after 1, 3, 15, 28, and 40 days. Functional parameters, such
The dromedary camel displays annual variation in hypothalamic kisspeptin and Arg-Phe-amide-related peptide-3 according to sex, season, and breeding activity
Ainani H, et al.
The Journal of Comparative Neurology, 528(1), 36-51 (2020)
K M Mader et al.
Genes, brain, and behavior, 7(7), 740-753 (2008-06-04)
Mucopolysaccharidosis type IIIA (MPS IIIA) is an inherited neurodegenerative lysosomal storage disorder characterised by progressive loss of learned skills, sleep disturbance and behavioural problems. Reduced activity of sulphamidase (SGSH; EC 3.10.1.1) results in intracellular accumulation of heparan sulphate (HS), with
J Balthazart et al.
Neuroscience, 153(4), 944-962 (2008-05-02)
Songbirds produce learned vocalizations that are controlled by a specialized network of neural structures, the song control system. Several nuclei in this song control system demonstrate a marked degree of adult seasonal plasticity. Nucleus volume varies seasonally based on changes

Artículos

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Questions

  1. What is the DAB volume in one set?

    1 answer
    1. As mentioned under 'Quantity', each DAB Liquid Substrate System will provide 250 mL of DAB substrate solution. The components of this kit are as follows:
      -10X DAB Liquid Chromogen 25 mL
      -Ready-to-use buffer/peroxide solution 250 mL

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