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14365C

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EX-CELL® CD CHO Fusion

Animal-component free, without L-glutamine, without hypoxanthine, without thymidine, liquid, sterile-filtered, suitable for cell culture

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

Nivel de calidad

500

esterilidad

sterile-filtered

Línea del producto

EX-CELL®

Formulario

liquid

técnicas

cell culture | mammalian: suitable (suspension)

componentes

L-glutamine: no

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

EX-CELL® CD CHO Fusion is a chemically defined, animal-component free medium developed for the long-term growth of Chinese Hamster Ovary (CHO) cells. The absence of any large macro molecules allows for isolation and purification of secreted proteins from the cells. This medium is supplied without L-glutamine to aid in media stability, to avoid L-glutamine degradation that causes ammonia build-up and to provide an appropriate medium for the culture of CHO cells using glutamine synthetase selection (example: MilliporeSigma’s CHOZN GS knockout cell line). This medium does not contain hypoxanthine or thymidine to allow for its use with dihydrofolate reductase(DHFR-) gene amplification systems (example: MilliporeSigma’s CHOZN DHFR knockout cell line).

Cantidad

Formulated to contain 20.9 grams of powder per liter of medium.

Información legal

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000343283
0000343283
0001586569
0000343265
0000343267

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Noriko Yamano-Adachi et al.
Scientific reports, 10(1), 17612-17612 (2020-10-21)
Chinese hamster (Cricetulus griseus) ovary-derived Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutics because of their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, on proteins.
Qiong Wang et al.
Biotechnology and bioengineering, 116(9), 2303-2315 (2019-05-08)
With the increasing demand to provide more detailed quality attributes, more sophisticated glycan analysis tools are highly desirable for biopharmaceutical manufacturing. Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the
Weiyi Liu et al.
Biotechnology progress, 37(1), e3061-e3061 (2020-08-05)
Antibody-dependent cellular cytotoxicity (ADCC) is the primary mechanism of actions for several marketed therapeutic antibodies (mAbs) and for many more in clinical trials. The ADCC efficacy is highly dependent on the ability of therapeutic mAbs to recruit effector cells such

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Optimizing CHO Cells for Higher Productivity

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CHO Media and Feeds for Optimized Production of Therapeutic Proteins

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