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I2752

Sigma-Aldrich

L-Isoleucina

≥98% (HPLC)

Sinónimos:

Ácido (2S,3S)-2-amino-3-metilpentanoico

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About This Item

Fórmula lineal:
C2H5CH(CH3)CH(NH2)CO2H
Número de CAS:
73-32-5
Peso molecular:
131.17
Beilstein/REAXYS Number:
1721792
EC Number:
200-798-2
MDL number:
MFCD00064222
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
24896006
NACRES:
NA.26
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Nombre del producto

L-Isoleucina, reagent grade, ≥98% (HPLC)

grade

reagent grade

Quality Level

200

assay

≥98% (HPLC)

form

powder

color

white

mp

288 °C (dec.) (lit.)

solubility

1 M NH4OH: 50 mg/mL

application(s)

detection

SMILES string

CC[C@H](C)[C@H](N)C(O)=O

InChI

1S/C6H13NO2/c1-3-4(2)5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t4-,5-/m0/s1

InChI key

AGPKZVBTJJNPAG-WHFBIAKZSA-N

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Application

L-Isoleucine has been used in capillary feeder (CAFE) assay in a study to determine the consumption of amino acids by Drosophila melanogaster.[1] It has also been used as one of the components of an amino acid mixture, which is used as a supplement for synthetic complete (SC) media.[2]

Biochem/physiol Actions

L-Isoleucine is an isomer of L-leucine[3] and is an essential amino acid.[4] It is synthesized from threonine[5] and is a branched-chain hydrophobic amino acid.[6][7]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000418184
0000418183
0000418183
0000418184
0000409719

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Conversion of ammonia or urea into essential amino acids,L-leucine,L-valine, andL-isoleucine using artificial cells containing an immobilized multienzyme system and dextran-NAD+
Applied Biochemistry and Biotechnology, 26 (1990)
Mated Drosophila melanogaster females consume more amino acids during the dark phase
Shun U
PLoS ONE (2017)
Large-scale Phenotypic Profiling of Gene Deletion Mutants in Candida glabrata
Fabian Istel
Bio-protocol (2015)
Amino Acid Biosynthesis Is Regulated by Feedback Inhibition
Biochemistry (5th Edition) null
Inhibitory effect of aroma on the bitterness of branched-chain amino acid solutions.
Mukai J
Chemical & Pharmaceutical Bulletin, 55(11) (2007)
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Chromatograms

application for HPLC

Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Is Leucine light-sensitive?

    1 answer

    1. According to literature data, L Isoleucine has not been reported to be sensitive to light. A relevant publication for reference is EFSA Journal 2010; 8(1):1425. It states that "L-isoleucine is known to be not particularly sensitive to light.

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