The solution stability of this specific product has not been determined, but solutions are quite stable. Product P4864, a 1 mg/mL prepared solution, is stored refrigerated and offers a warranty of 1 year from the date of shipment.
P4170
Yoduro de propidio
≥94.0% (HPLC), fluorescent dye, powder
Sinónimos:
Diyoduro de 3,8-diamino-5-[3-(dietilmetilamonio)propil]-6-fenilfenantridinio
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
10 mg | Fecha estimada de envío07 de septiembre de 2026desdePUDAHUEL | $66.600 |
25 mg | Comprobar disponibilidad del carrito | $125.000 |
100 mg | Comprobar disponibilidad del carrito | $174.000 |
250 mg | Comprobar disponibilidad del carrito | $378.000 |
500 mg | Comprobar disponibilidad del carrito | $689.000 |
1 g | Comprobar disponibilidad del carrito | $1.460.000 |
Acerca de este artículo
Fórmula empírica (notación de Hill):
C27H34I2N4
Número CAS:
Peso molecular:
668.39
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
41106305
EC Number:
247-081-0
MDL number:
Beilstein/REAXYS Number:
3843838
Assay:
≥94.0% (HPLC)
Form:
powder
Servicio técnico
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Permítanos ayudarleNombre del producto
Yoduro de propidio, ≥94.0% (HPLC)
Quality Segment
assay
≥94.0% (HPLC)
form
powder
mp
220-225 °C (dec.) (lit.)
storage temp.
2-8°C
SMILES string
[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI key
XJMOSONTPMZWPB-UHFFFAOYSA-M
General description
El yoduro de propidio (PI) es un colorante fluorescente quepuede utilizarse para teñir los ácidos nucleicos. Es impermeable en células viables ointactas. Puede penetrar fácilmente en células muertas o moribundas o con la integridad de la membrana afectada. El PI tiene la capacidad de unirse fácilmente al ADN.
Application
Features and Benefits
Este compuesto es un producto destacado para la investigación de la apoptosis. Pulse aquí para descubrir más productos destacados para apoptosis. Encontrará más información sobre moléculas pequeñas bioactivas para otras áreas de investigación en sigma.com/discover-bsm.
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Este artículo | |||
|---|---|---|---|
| description ≥94.0% (HPLC) | description Membrane impermeable DNA intercalator. | description ≥94% (HPLC) | description A convenient form of propidium iodide useful for flow cytometry studies. |
| form powder | form solid | form powder | form liquid |
| assay ≥94.0% (HPLC) | assay - | assay ≥94% (HPLC) | assay - |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mp 220-225 °C (dec.) (lit.) | mp - | mp 220-225 °C (dec.) (lit.) | mp - |
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signalword
Warning
Clase de almacenamiento
11 - Combustible Solids
ppe
dust mask type N95 (US), Eyeshields, Gloves
hcodes
Hazard Classifications
Eye Irrit. 2 - Muta. 2
flash_point_f
No data available
flash_point_c
No data available
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How long can we stored PI dissolved in water?
1 answer-
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What is the procedure for dissolving this product?
1 answer-
Propidium iodide is soluble in water at a concentration of 1 mg/mL.
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Is it necessary to carry out an RNase treatment before staining with propidium iodide in my immunofluorescence assay on insect tissue fixed in 4% paraformaldehyde? Additionally, is there the complete staining protocol for propidium iodide in immunofluorescence using a confocal microscope?
1 answer-
The product has not been validated for immunofluorescence assay in our laboratory to establish a reference protocol. In cases where propidium iodide binds to both DNA and RNA, the use of RNase is common to differentiate DNA staining from undesired RNA staining. For an application protocol, one may refer to a citation of this product in an immunofluorescence assay that utilizes RNase to visualize DNA-specific staining. The citation is as follows: J Cancer. 2020 Mar 5;11(11):3274-3287. The article discusses the dual role of BI 2536, a small-molecule inhibitor targeting PLK1, in inducing apoptosis and attenuating autophagy in neuroblastoma cells. The PMID for the article is 32231733.
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Is this reagent suited for viability assays at FACS?
1 answer-
While this product is not tested for any specific application, propidium iodide is commonly used in cell-based assays such as FACS. Please see the link below to review publications detailing the use of this item in flow cytometry:
https://scholar.google.com/scholar?hl=en&as_sdt=0%2C22&q=%22p4170%22+flow+cytometry&btnG=Helpful?
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What is the method to dissolve this product?
1 answer-
Propidium iodide can be dissolved in water at a concentration of 1 mg/mL. Please see the sample Certificate of Analysis for solubility information: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P4170-BULKWXBF1544V.pdf
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