11412272001
Roche
Transforming Growth Factor-β1, human (hTGF-β1)
recombinant (CHO cells)
Sinónimos:
hTGF
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About This Item
Código UNSPSC:
12352207
Productos recomendados
origen biológico
human
Nivel de calidad
recombinante
expressed in CHO cells
Ensayo
>95% (SDS-PAGE)
Formulario
solution
potencia
<0.1 ng/mL EC50
mol peso
25,000 Da
envase
pkg of 1 mL (1 μg)
fabricante / nombre comercial
Roche
condiciones de almacenamiento
avoid repeated freeze/thaw cycles
impurezas
<0.1 EU/μgtested (LAL test)
Nº de acceso UniProt
temp. de almacenamiento
−20°C
Información sobre el gen
human ... TGFB1(7040)
Descripción general
Recombinant, human TGF- β1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques.
Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane.
Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane.
Especificidad
Species specificity: Transforming growth factor-β1 (TGF-β1) is effective on human and mouse cells.
Specific activity/EC50: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.
Specific activity/EC50: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.
Aplicación
Transforming growth factor-β1, human (hTGF-β1) has been used to explore function and underlying mechanism of periostin (PN) during orthodontic periodontal ligament remodeling.
Acciones bioquímicas o fisiológicas
Transforming Growth Factor-β1, human (hTGF-β1), is a multifunctional growth factor affecting many functions in a variety of different cells.
Transforming growth factor-β (TGF-β) family comprises of a group of multifunctional cytokines which are involved in controlling apoptosis, growth and differentiation of cells, and play important role during embryo development.
Calidad
Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(Note: 1 EU corresponds to 0.1 ng)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(Note: 1 EU corresponds to 0.1 ng)
Secuencia
Chain Length 2 x 112 AA
Two identical polypeptide chains (112 amino acids) connected by disulfide bridges, identical to natural hTGF-β1.
Definición de unidad
EC50 definition: The concentration of hTGF-β1 that is required to mediate half maximal inhibition of DNA synthesis (BrdU incorporation) with Mv1-Lu cells.
Nota de preparación
Working concentration: 0.01 - 10 ng/ml
Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.
Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.
Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.
Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.
Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Otras notas
For life science research only. Not for use in diagnostic procedures.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
does not flash
Punto de inflamabilidad (°C)
does not flash
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