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Key Documents

AP501P

Sigma-Aldrich

Goat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, HRP conjugate, Species Adsorbed

Chemicon®, from goat

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable

shipped in

dry ice

target post-translational modification

unmodified

General description

IgG is the most abundant immunoglobulin compose of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Specificity

Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.
The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Immunogen

Epitope: Mouse IgM, IgG, IgA
Prepared from pooled antisera from goats hyperimmunized with mouse IgM, IgG and IgA paraproteins.

Application

ELISA: 1:4,000-1:8,000

Optimal working dilutions must be determined by the end user.
Goat anti-Mouse IgM+IgG+IgA (H+L) Antibody, HRP conjugate, Species Adsorbed detects level of Mouse IgM+IgG+IgA (H+L) & has been published & validated for use in ELISA.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Physical form

Affinity Chromatography
Purified by affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked to agarose. Liquid in 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4.

Storage and Stability

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Store long-term at -20°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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Marlena M Westcott et al.
Microorganisms, 11(8) (2023-08-26)
Inactivated whole-cell vaccines present a full repertoire of antigens to the immune system. Formalin treatment, a standard method for microbial inactivation, can modify or destroy protein antigenic epitopes. We tested the hypothesis that photochemical inactivation with psoralen and UVA light

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