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AB5882

Sigma-Aldrich

Anti-IP3 Receptor 1 Antibody

Chemicon®, from rabbit

Sinónimos:

Inositol 1,4,5-Trisphosphate Receptor 1, ITPR1

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

affinity purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

polyclonal

purificado por

affinity chromatography

reactividad de especies

rat

fabricante / nombre comercial

Chemicon®

técnicas

western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... ITPR1(3708)

Especificidad

Recognizes IP3 Receptor 1. The epitope does not share homology with any other known proteins.

Inmunógeno

Synthetic peptide corresponding amino acids 2732-2750 of rat IP3R1 (Accession number P29994). It is expected that the antibody will also react with mouse and human due to identical sequence homology.

Aplicación

Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-IP3 Receptor 1 Antibody is validated for use in WB for the detection of IP3 Receptor 1.
Western blot: 1:200-1:1,000 using ECL on rat brain membranes.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Forma física

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg fo

Almacenamiento y estabilidad

Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Nota de análisis

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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David Krizaj et al.
The Journal of comparative neurology, 475(4), 463-480 (2004-07-06)
Changes in intracellular calcium concentration, [Ca2+]i, modulate the flow of visual signals across all stages of processing in the retina, yet the identities of Ca2+ transporters responsible for these changes are still largely unknown. In the current study, the distribution
Ian F Smith et al.
Biophysical journal, 107(4), 834-845 (2014-08-21)
Puffs are local Ca(2+) signals that arise by Ca(2+) liberation from the endoplasmic reticulum through the concerted opening of tightly clustered inositol trisphosphate receptors/channels (IP3Rs). The locations of puff sites observed by Ca(2+) imaging remain static over several minutes, whereas
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PloS one, 12(1), e0169861-e0169861 (2017-01-11)
Anoxia is one of the most prevalent causes of neonatal morbidity and mortality, especially in preterm neonates, constituting an important public health problem due to permanent neurological sequelae observed in patients. Oxygen deprivation triggers a series of simultaneous cascades, culminating
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Regulation of IP3 receptors (IP3Rs) by IP3 and Ca2+ allows regenerative Ca2+ signals, the smallest being Ca2+ puffs, which arise from coordinated openings of a few clustered IP3Rs. Cells express thousands of mostly mobile IP3Rs, yet Ca2+ puffs occur at

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