13-110
MBP, desfosforilado
Sinónimos:
MBP protein
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About This Item
UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.41
Productos recomendados
biological source
bovine
Quality Level
form
liquid
shelf life
2 yr
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable
solubility
water: soluble
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
bovine ... MBP(618684)
General description
Research Area: Cell Signaling
Myelin basic protein (MBP) constitutes approximately 30% of the total protein and accounts for about 10% of the dry weight of myelin. It is primarily associated with the cytosolic side of the oligodendrocyte (OL) membrane, largely through electrostatic interactions with acidic lipids. This protein is distributed throughout compact internodal myelin. MBP consists of multiple post-translationally modified isomers with varying charges, some of which arise from phosphorylation at several sites by different kinases, including mitogen-activated protein kinase (MAPK).
Myelin basic protein (MBP) constitutes approximately 30% of the total protein and accounts for about 10% of the dry weight of myelin. It is primarily associated with the cytosolic side of the oligodendrocyte (OL) membrane, largely through electrostatic interactions with acidic lipids. This protein is distributed throughout compact internodal myelin. MBP consists of multiple post-translationally modified isomers with varying charges, some of which arise from phosphorylation at several sites by different kinases, including mitogen-activated protein kinase (MAPK).
Application
Proteína básica de mielina purificada del cerebro bovino y desfosforilada usando la proteína fosfatasa Lambda. No se detecta fosforilación endógena, determinada por inmunotransferencia de 1 microgramo de MBP con 1 microgramo/ml de anti fosfo-MBP.
Biochem/physiol Actions
Myelin basic protein (MBP) is distributed throughout compact internodal myelin. It binds to negatively charged lipids on the cytosolic surface of oligodendrocyte membranes, likely facilitating the adhesion of these surfaces in the multilayered myelin sheath. MBP is characterized as an intrinsically unstructured protein, providing it with the flexibility to bind to a charged surface along its entire length and adapt its local conformation as needed to optimize binding to various targets. Such proteins often function as multifunctional regulatory proteins.
MBP interacts with polyanions such as actin filaments and microtubules in vitro and binds to other ligands such as Ca2+-calmodulin (CaM). MBP binds to G-actin, inducing its polymerization, and it bundles actin filaments. It also polymerizes actin, bundles F-actin filaments, and binds actin filaments to lipid bilayers through electrostatic interactions. Phosphorylation and dephosphorylation of MBP occur in the myelin sheath in response to electrical activity in the brain.
MBP interacts with polyanions such as actin filaments and microtubules in vitro and binds to other ligands such as Ca2+-calmodulin (CaM). MBP binds to G-actin, inducing its polymerization, and it bundles actin filaments. It also polymerizes actin, bundles F-actin filaments, and binds actin filaments to lipid bilayers through electrostatic interactions. Phosphorylation and dephosphorylation of MBP occur in the myelin sheath in response to electrical activity in the brain.
Quality
Evaluada habitualmente como sustrato en un ensayo de cinasa utilizando MAP cinasa 2/Erk2 (nº de referencia 14-173)
Physical form
MOPS 10mM, pH 7,0, MnCl2 128 mM, EDTA 641mM, 1,134μg de fosfatasa lambda inactiva y azida sódica al 0,05 %
Storage and Stability
2 años a -20°C
Other Notes
Este producto es de origen bovino. La exportación de este producto a determinados países puede estar restringida. Póngase en contacto con el servicio de atención al cliente o con su distribuidor local para obtener información sobre la disponibilidad del producto y las opciones de exportación.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Salvo que se indique lo contrario en nuestro catálogo o en otra documentación de la empresa que acompañe al producto, nuestros productos están indicados sólo para investigación y no deben utilizarse para ningún otro propósito, como, entre otros, usos comerciales no autorizados, diagnóstico in vitro, usos terapéuticos ex vivo o in vivo o cualquier tipo de consumo o aplicación en humanos o animales.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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