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P1585

PMA

for use in molecular biology applications, ≥99% (HPLC), Molecular Biology

Sinónimos:

Forbol 12-miristato 13-acetato, Aceite de crotón, Activador de la PKC, Activador de la proteína cinasa C, Activador del NF-κB, Éster de forbol, 12-O-Tetradecanoilforbol 13-acetato, 4β,9α,12β,13α,20-Pentahidroxitiglia-1,6-dien-3-ona 12-tetradecanoato 13-acetato, PMA, TPA

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Talla/SKUDisponibilidadPrecio
1 mg
Comprobar disponibilidad del carrito
$227.000
5 mg
Comprobar disponibilidad del carrito
$240.000
10 mg
Comprobar disponibilidad del carrito
$897.000
25 mg
Comprobar disponibilidad del carrito
$1.180.000

Acerca de este artículo

Fórmula empírica (notación de Hill):
C36H56O8
Número CAS:
16561-29-8
Peso molecular:
616.83
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
24898219
MDL number:
MFCD00036736
Beilstein/REAXYS Number:
2407201
Assay:
≥99% (HPLC)
Form:
(Lyophilisate)
Storage condition:
protect from light

$227.000

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grade

Molecular Biology

Quality Segment

assay

≥99% (HPLC)

form

(Lyophilisate)

mol wt

616.83  g/mol

storage condition

protect from light

solubility

DMSO: soluble (DMSO solutions can be stored dark at −20 °C for at least six months.), ethanol: soluble (Dissolve in water-miscible organic solvent prior to preparing aqueous solutions)

storage temp.

−20°C

SMILES string

CCCCCCCCCCCCCC(=O)O[C@@H]1[C@@H](C)[C@@]2(O)C(C=C(CO)C[C@@]3(O)C2C=C(C)C3=O)C4C(C)(C)[C@]14OC(C)=O

InChI

1S/C36H56O8/c1-7-8-9-10-11-12-13-14-15-16-17-18-29(39)43-32-24(3)35(42)27(30-33(5,6)36(30,32)44-25(4)38)20-26(22-37)21-34(41)28(35)19-23(2)31(34)40/h19-20,24,27-28,30,32,37,41-42H,7-18,21-22H2,1-6H3/t24-,27+,28-,30-,32-,34-,35-,36-/m1/s1

InChI key

PHEDXBVPIONUQT-RGYGYFBISA-N

General description

El forbol 12-miristato 13-acetato (PMA) puede inducir la actividad de la PLD1 (fosfolipasa D1), pero no de la PLD2 (fosfolipasa D2).

A través de la vía de comunicación de la proteína cinasa C (PKC), el PMA es un promotor tumoral asociado con:

  • la transcripción génica
  • el crecimiento celular
  • la diferenciación celular
  • la muerte celular programada
  • la vía inmunitaria
  • la desensibilización de receptores

El forbol 12-miristato 13-acetato se utiliza para estudiar:

  • los factores de crecimiento
  • las hormonas
  • la regulación del crecimiento y diferenciación celulares mediada por citocinas
El PMA (forbol 12-miristato 13-acetato) es un activador de la proteína cinasa C (PKC) y un promotor tumoral conocido. El PMA exhibe también actividad clastogénica (rotura del cromosoma).

Application

Adecuado para
  • estudios de biología molecular como patrón
  • estudios analíticos de promotores tumorales
  • promoción del crecimiento celular
El PMA se ha utilizado:
  • para la detección de citocinas intracelulares
  • en ensayos de estrés oxidativo CellROX
  • para la estimulación de células Huh7 para investigar la re-distribución de la dipeptidil peptidasa 9 (DPP9)
  • como control positivo en la estimulación del receptor de las células B (BCR) de las células de leucemia linfocítica crónica (CLL)
  • en la diferenciación de los monocitos (THP-1) en macrófagos

Biochem/physiol Actions

Potente promotor tumoral; activa la proteína cinasa C (PKC) in vivo e in vitro.

Features and Benefits

  • Estimulación de la producción de interleucina 2 de las células Jurkat
  • Estimulación de las células T
  • Activador de la proteína cinasa C (PKC)
  • Activación del NF-κB
  • Promoción del crecimiento celular
  • Utilizada en estudios analíticos de promotores tumorales
  • Calidad para biología molecular

Analysis Note

  • Estimulación de la producción de interleucina 2 de las células Jurkat
  • Estimulación de las células T
  • Activador de la proteína cinasa C (PKC)
  • Activación del NF-κB
  • Promoción del crecimiento celular
  • Utilizada en estudios analíticos de promotores tumorales
  • Calidad para biología molecular

Disclaimer

Fotosensible, proteger de la luz.

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Este artículo
79346P8139524400
PMA for use in molecular biology applications, ≥99% (HPLC), Molecular Biology

Sigma-Aldrich

P1585

PMA

Forbol 12-miristato 13-acetato synthetic, ≥98.0% (TLC)

Sigma-Aldrich

79346

Forbol 12-miristato 13-acetato

Forbol 12-miristato 13-acetato ≥99% (TLC), film or powder

Sigma-Aldrich

P8139

Forbol 12-miristato 13-acetato

Phorbol-12-myristate-13-acetate Phorbol-12-myristate-13-acetate, CAS 16561-29-8, is the most common phorbol ester. Activates PKC at nanomolar concentrations.

Sigma-Aldrich

524400

Phorbol-12-myristate-13-acetate

description

for use in molecular biology applications, ≥99% (HPLC), Molecular Biology

description

synthetic, ≥98.0% (TLC)

description

≥99% (TLC), film or powder

description

Phorbol-12-myristate-13-acetate, CAS 16561-29-8, is the most common phorbol ester. Activates PKC at nanomolar concentrations.

form

(Lyophilisate)

form

film, powder or solid (or crystals)

form

film or powder

form

solid

assay

≥99% (HPLC)

assay

≥98.0% (TLC)

assay

≥99% (TLC)

assay

≥98% (HPLC)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

616.83  g/mol

mol wt

-

mol wt

-

mol wt

-

solubility

DMSO: soluble (DMSO solutions can be stored dark at −20 °C for at least six months.), ethanol: soluble (Dissolve in water-miscible organic solvent prior to preparing aqueous solutions)

solubility

chloroform: 10 mg/mL, clear, colorless to light yellow

solubility

ethanol: soluble (Dissolve in water-miscible organic solvent prior to preparing aqueous solutions.), DMSO: soluble

solubility

DMSO: 10 mg/mL

Palabra de advertencia

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 2 Oral - Carc. 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Clase de almacenamiento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

¿Qué está pasando?

WGK 3

EPI (equipos de protección individual)

dust mask type N95 (US), Eyeshields, Gloves

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Lot/Batch Number

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Questions

1–10 of 14 Questions  

  1. How can I safely remove the metal ring from the bottle?

    1 answer

    1. This product is packaged in amber glass serum vials with a septum and aluminim crimp cap.

      The perforated center tab of the crimp cap may be removed manually to reveal the septum, through which the material may be reconstituted via needle and syringe.

      The remaining aluminum 'ring' in intended to remain in place to secure the septum.

      Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/311/794/p1585dat.pdf

      Helpful?

  2. Can I use PMA alone for T cell activation or do I need to add Ionomycin as well?

    1 answer

    1. This product is tested for suitabilty for T-Cell activation without the addition of Ionomycin. However lectin/PHA is added. While not strictly required, Ionomycin is cited as an additional component in a number of publications. Please see the link below to review one such example:
      https://pmc.ncbi.nlm.nih.gov/articles/PMC10326540/

      Please see the following link to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/311/794/p1585dat.pdf

      Helpful?

  3. Can I store the stock solutions in DMSO at -80°C?

    1 answer

    1. For this product, stock solutions prepared in Dimethyl sulfoxide (DMSO) can be stored as aliquots at -20°C in the dark for at least six months. The stability of solutions stored at -80°C has not been evaluated. Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/311/794/p1585dat.pdf

      Helpful?

  4. I received the product of PMA cat# P1585 in solution. But what is the product of PMA cat#1585 concentration? or volume? without concentration or volume can't be calculate the further dilution.

    1 answer

    1. The stock concentration is 100ug/mL.

      Helpful?

  5. How can I resuspend properly the PMA into the shipped vial? Can I open the vial?

    1 answer

    1. The packaging consists of an amber serum vial sealed with a septum and an aluminum crimp cap. The product is typically dissolved in the vial by injecting the appropriate volume of the selected solvent through the septum using a syringe. Additional solubility and solution stability information can be reviewed in the datasheet available for the general use product, P8139. See the link below to review these recommendations:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/816/596/p8139pis.pdf

      Helpful?

  6. What is the stock concentration of the PMA when adding DMSO. and How much of DMSO do we add?

    1 answer

    1. Suitability Assay - 2.5 ml of Jurkat cells (1 x 10e6 cells/mL) and 2.5 mLof fresh media (RPMI 1640 + 10% fetal calf serum containing 10 ml/l penicillin-streptomycin) were added to 25 cm2 culture bottles.
      The following additions were made in duplicate.
      a. Control - no additions
      b. 1 µg/ml PHA Control - add 10 µl PHA stock solution (0.5 mg/ml PHA in filter-sterilized PBS)
      c. 1 µg/ml PHA + 50 ng/ml PMA – 10 µl PHA stock solution + 2.5 µl PMA stock solution (100 µg/ml PMA in DMSO).

      As stated in part c. - the PMA stock solution is 100µg/ml PMA in DMSO.

      Please see the datasheet for more details:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/311/794/p1585dat.pdf

      Helpful?

  7. What is the storage temperature for Phorbol 12-myristate 13-acetate, Product P1585?

    1 answer

    1. Phorbol 12-myristate 13-acetate should be stored at -20°C.

      Helpful?

  8. What is the difference between Phorbol 12-myristate 13-acetate, Product P8139 and molecular biology grade Product P1585?

    1 answer

    1. Product P1585 has been tested and found suitable for stimulation of interleukin 2 production of Jurkat cells. Product P8139 has not tested in this application.

      Helpful?

  9. What is the stability of stock solutions of Phorbol 12-myristate 13-acetate, Product P1585, and how should I store them?

    1 answer

    1. Stock solutions in DMSO can be stored in the dark as aliquots at -20°C for at least six months.

      Helpful?

  10. How is Product P1585, Phorbol-12-myristate-13-acetate, packaged?

    1 answer

    1. P1585-1MG, P1585-10MG and P1585-25MG come to us pre-packaged by our supplier; all three sizes are packaged the same way. The packaging consists of an amber serum vial with a septum and an aluminum crimp cap. The septum has a PTFE (Teflon) liner, so the material inside the vial could only come in contact with glass and Teflon.

      Helpful?

1–10 of 14 Questions  

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