During Quality Control, no testing to performed involving confocal microscopy. Conjugate 5-aminofluorescein (AFL)-human serum albumin has been used to stain brain cells, although it does not appear confocal microscopy was performed. Below is the reference: https://www.sigmaaldrich.com/tech-docs/paper/250406
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 g | Comprobar disponibilidad del carrito | $278.000 |
5 g | Comprobar disponibilidad del carrito | $958.000 |
Acerca de este artículo
form
powder
Quality Segment
mp
223 °C (dec.) (lit.)
solubility
methanol: 5 mg/mL
λmax
490-501 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Nc1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
InChI
1S/C20H13NO5/c21-10-1-4-14-13(7-10)19(24)26-20(14)15-5-2-11(22)8-17(15)25-18-9-12(23)3-6-16(18)20/h1-9,22-23H,21H2
InChI key
GZAJOEGTZDUSKS-UHFFFAOYSA-N
General description
Application
1 of 1
Este artículo | |||
|---|---|---|---|
| description - | description ≥90% (HPLC) | description used as stain for amyloid | description ≥90% (HPLC) |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| solubility methanol: 5 mg/mL | solubility DMSO: 5 mg/mL | solubility methanol: 1 mg/mL | solubility chloroform: 10 mg/mL, dark red |
| form powder | form powder | form powder | form powder |
| storage temp. room temp | storage temp. −20°C | storage temp. room temp | storage temp. room temp |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| mp 223 °C (dec.) (lit.) | mp - | mp - | mp 252-254 °C (lit.) |
Palabra de advertencia
Warning
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Órganos diana
Respiratory system
Clase de almacenamiento
11 - Combustible Solids
¿Qué está pasando?
WGK 3
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
EPI (equipos de protección individual)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Can this be used to stain cells and visualize them under the confocal microscope?
1 answer-
Helpful?
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Dear manager, I want to ask you 2 questions about this product. 1. Is it a flurescence dye? Can you show me its adsorption or emission spectrum? 2. Is the amine group reactive?
1 answer-
Yes, Fluoresceinamine, isomer I is classified as belonging to a group of derivatized fluorosceins. The product is fluorescent. Although no quality control testing is performed to check for excitation and emission, the dye is expected to excite around 490 nm and emit around 520 nm.
As the H2N- group is not acetylated or protonated, the amine group can be considered reactive. There is literature available stating Fluoresceinamine, isomer I can be covalently bound to human serum albumin.
Helpful?
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What is the solubility of Fluoresceinamine, isomer I, CAS 3326-34-9?
1 answer-
Soluble in methanol at a concentration of 5 mg/mL. Soluble in DMSO at a concentration of ≥ 32 mg/mL.
Helpful?
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