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TOYOPEARL® DEAE-650M Bulk Media

phase DEAE (diethylaminoethyl), bottle of 250 mL, 65 μm particle size

Sinonimo/i:

TOYOPEARL® DEAE-650M Bulk Media

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About This Item

Codice UNSPSC:
47101511

Descrizione

DEAE-650M

Forma fisica

slurry

Caratteristiche

counter ion Cl-

Confezionamento

bottle of 250 mL

Parametri

3 bar max. pressure

tecniche

HPLC: suitable

Matrice

methacrylate

Gruppo funzionale matrice

DEAE (diethylaminoethyl) phase

Dimensione particelle

65 μm

pH di lavoro

2-10

Capacità

0.10 meq/mL±0.02 meq/mL ion exchange capacity
 30 g/L±5 adsorption capacity (BSA)
 30 mg/mL±5 mg/mL binding capacity (bovine serum albumin)

Compatibilità

mode of use weak anion exchange chromatography

Tecnica di separazione

anion exchange

Descrizione generale

Toyopearl DEAE-650 resin is a weak anion exchanger for blood fractionation and other biomolecule purification. It can be used for high throughput capture, intermediate purification, and polishing process steps. If more powerful resolution is needed TSKgel DEAE-5PW resin should be used. Selectivity remains the same.

Applicazioni

TOYOPEARL® DEAE-650M is used in separation media and ion exchange resins. TOYOPEARL® DEAE-650M has been used in haemophilia studies.

Specifiche

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Stato fisico

Shipped in 20% (v/v) ethanol.

Prodotti consigliati

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Note legali

Toyopearl is a registered trademark of Tosoh Corporation

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Classi di pericolo

Flam. Liq. 3

Classe di pericolosità dell'acqua (WGK)

WGK 1

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
107H0708

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Sigma-Aldrich

A50120

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GE17-0709-10

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DEAE–Sepharose™ CL-6B

Sigma-Aldrich

DCL6B100

DEAE–Sepharose™

DEAE–Sephadex® A-25 chloride form

Sigma-Aldrich

A25120

DEAE–Sephadex® A-25 chloride form

Q Sepharose™ Fast Flow Cytiva 17-0510-01, pack of 300 mL

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DEAE Sepharose™ Fast Flow Cytiva 17-0709-01, pack of 500 mL

GE17-0709-01

DEAE Sepharose™ Fast Flow

Günter Iberer et al.
Journal of chromatography. A, 972(1), 115-129 (2002-10-25)
Preparative continuous annular chromatography, a method to separate proteins in a truly continuous manner, was investigated in an industrial environment. Plasma-derived clotting factor IX concentrate was used as model protein. Separation of vitronectin, a common impurity in commercial available factor
P R Foster et al.
Vox sanguinis, 86(2), 92-99 (2004-03-17)
The risk of haemophiliacs contracting variant Creutzfeldt-Jakob disease (vCJD) via treatment with factor VIII concentrates is not known. Therefore, in order to determine the extent to which the vCJD agent might be removed during the preparation of factor VIII concentrate
Christine Machold et al.
Journal of biotechnology, 117(1), 83-97 (2005-04-16)
Two different approaches of matrix assisted refolding have been evaluated and compared to conventional refolding by dilution. Bovine alpha-lactalbumin was used for the studies as model protein. It was adsorbed under denaturing conditions on an ion exchange matrix and refolding
Masahiko Takanashi et al.
Journal of bioscience and bioengineering, 97(1), 78-81 (2005-10-20)
D(-)-3-hydroxybutyrate dehydrogenase (BDH; EC 1.1.1.30) from a poly(D(-)-3-hydroxybutyrate) (PHB) degrading bacterium, Acidovorax sp. SA1, was purified using Toyopearl DEAE-650M, red-Sepharose CL-4B, and Q Sepharose FF. The molecular mass of the enzyme was estimated as 27 kDa by SDS-PAGE and 110

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