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Merck

DFF100

DEAE–Sepharose

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Sinonimo/i:

Diethylaminoethyl–Sepharose

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50 ML

CHF 236.00

100 ML

CHF 391.00

CHF 236.00


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Informazioni su questo articolo

Numero CAS:
UNSPSC Code:
47101511
NACRES:
NA.56
MDL number:

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form

suspension

technique(s)

affinity chromatography: suitable

matrix

6% cross-linked agarose

bead size

45-165 μm (wet)

pore size

~4,000,000 Da exclusion limit

pH

2—12

capacity

110-160 μeq/mL binding capacity(gel volume)

Quality Level

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A50120DCL6B100CCF100
pore size

~4,000,000 Da exclusion limit

pore size

~200,000 Da exclusion limit

pore size

~4,000,000 Da exclusion limit

pore size

4,000,000 exclusion limit (av. mol. wt.)

matrix

6% cross-linked agarose

matrix

cross-linked dextran

matrix

6% cross-linked agarose

matrix

6% cross-linked agarose

technique(s)

affinity chromatography: suitable

technique(s)

protein purification: suitable

technique(s)

affinity chromatography: suitable

technique(s)

affinity chromatography: suitable

capacity

110-160 μeq/mL binding capacity(gel volume)

capacity

3-4 meq/g binding capacity

capacity

130-170 μeq/mL binding capacity (gel volume)(gel volume)

capacity

90-130 μeq/mL(gel basis)

form

suspension

form

powder

form

suspension

form

suspension

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

DFF100-500Ml′s update product number is GE17-0709-01

Application

DEAE-Sepharose has been used in:
  • anion exchange chromatography
  • to screen polyisoprenyl phosphate phosphatase activity[1]
  • to purify isoinhibitors[2]
  • for the purification of human immunodeficiency virus (HIV)
  • glycoprotein envelope (gp140 env)[3]

DEAE-Sepharose is used in affinity chromatography, protein chromatography and ion exchange chromatography. DEAE-Sepharose has been used to study pathogenesis of human disease and to develop a new assay for detecting the toxins of pathogenic strains of Clostridium difficile.

Biochem/physiol Actions

Diethylaminoethyl-sepharose (DEAE-sepharose) is a strong anion exchange column, where DEAE covalently binds to sepharose.[4]

Legal Information

Sepharose is a trademark of Cytiva

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Descrizione
Determinazione del prezzo

pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

Classe di stoccaggio

3 - Flammable liquids

wgk

WGK 1

flash_point_f

100.4 - 109.4 °F

flash_point_c

38 - 43 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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G L Yewey et al.
Biochemical and biophysical research communications, 148(3), 1520-1526 (1987-11-13)
Bovine heart cytochrome c oxidase, depleted of polypeptide subunits by alkaline detergent treatment, was characterized with respect to metal content, optical spectral properties, and oxidase activity. Treatment with 1.0% Triton X-100 at pH 9.5 followed by anion-exchange chromatography caused removal
Jessie Fernandez et al.
mBio, 12(1) (2021-02-11)
Rice blast disease caused by Magnaporthe oryzae is a devastating disease of cultivated rice worldwide. Infections by this fungus lead to a significant reduction in rice yields and threats to food security. To gain better insight into growth and cell
T M Schmidt et al.
Applied and environmental microbiology, 55(10), 2607-2612 (1989-10-01)
Luminescence of batch cultures of Xenorhabdus luminescens was maximal when cultures approached stationary phase; the onset of in vivo luminescence coincided with a burst of synthesis of bacterial luciferase, the enzyme responsible for luminescence. Expression of luciferase was aldehyde limited
Advances in Potato Chemistry and Technology (2016)
Rapid high-level production of functional HIV broadly neutralizing monoclonal antibodies in transient plant expression systems
Rosenberg Y, et al.
Testing, 8(3), e58724-e58724 (2013)

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