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53826-U

Supelco

Ascentis® Express 90 Å C18 (2.7 μm) HPLC Columns

L × I.D. 5 cm × 4.6 mm, HPLC Column

Sinonimo/i:

Core-shell (SPP) Fused Core C18 HPLC column

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About This Item

Codice UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Prezzi e disponibilità al momento non sono disponibili

Nome del prodotto

Colonna per HPLC Ascentis® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 5 cm × 4.6 mm

Materiali

stainless steel column

agenzia

suitable for USP L1

Nome Commerciale

Ascentis®

Caratteristiche

endcapped

Produttore/marchio commerciale

Ascentis®

Confezionamento

1 ea of

Parametri

60 °C temp. range
600 bar max. pressure (9000 psi)

tecniche

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

Lungh. × D.I.

5 cm × 4.6 mm

Area superficiale

135 m2/g

Impurezze

<5 ppm metals

Matrice

Fused-Core particle platform
superficially porous particle

Gruppo funzionale matrice

C18 (octadecyl) phase

Dimensione particelle

2.7 μm

Dimensione pori

90 Å

pH di lavoro

2-9

applicazioni

food and beverages

Tecnica di separazione

reversed phase

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Categorie correlate

Descrizione generale

Le colonne per HPLC Ascentis Express, grazie alla tecnologia delle particelle Fused-Core®, offrono l′alta velocità e l′elevata efficienza delle particelle di dimensioni inferiori a 2 μm, mantenendo ridotte nel contempo le contropressioni. La combinazione di alta efficienza e bassa contropressione è un grande vantaggio per tutti coloro che utilizzano la UPLC® (o altri sistemi a pressione ultra-alta) e l′HPLC convenzionale.
Per ulteriori informazioni su questa nuova tecnologia, si rimanda alla pagina Ascentis Express

Applicazioni

Analisi HPLC delle capsaicine contenute nel peperoncino con la colonna Ascentis® Express C18, 2,7 μm
Analisi UHPLC/MS dell′olio di mais con la colonna Ascentis® Express C30 confrontata con Ascentis® Express C18
Analisi UHPLC del paracetamolo e delle sue impurezze con la colonna Ascentis® Express C18, 2,7 μm
Analisi HPLC dei parabeni con la colonna Ascentis®Express C18, 2,7 μm

Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters

Applicazioni

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Prodotti correlati

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Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H317

Consigli di prudenza

P280

Classi di pericolo

Skin Sens. 1

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
USUT007393
USUT007392
USUT007391
USUT007390
USUT007389

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Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection.

Untargeted profiling of Glycyrrhiza glabra extract with 2D LC MS

An RP-LC×RP-LC-PDA-MS method for the metabolic profiling of G. glabra extract using the MSG approach for enhanced metabolic coverage.

Derivatization and Separation of Aliphatic Amines

Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.

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Chromatograms

application for HPLC, application for SPEapplication for HPLCapplication for HPLCapplication for HPLC, application for SPE

Questions

1–9 of 9 Questions  

  1. How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?

    1 answer

    1. The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.

      Helpful?

  2. What flow rate should I use with Ascentis® Express HPLC Columns?

    1 answer

    1. Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;  0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Can Ascentis® Express HPLC Columns be used for LC-MS?

    1 answer

    1. Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.

      Helpful?

  5. Is there anything special I need to do to my HPLC system to use Ascentis Express?

    1 answer

    1. Nothing special is required to use Ascentis Express HPLC columns.  To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level.  For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).

      Helpful?

  6. Can I use Ascentis Express on a UHPLC system?

    1 answer

    1. Yes.  Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

      Helpful?

  7. Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?

    1 answer

    1. While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.

      Helpful?

  8. Can I use Ascentis Express on any type of HPLC system?

    1 answer

    1. Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.  Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.

      Helpful?

  9. What is the carbon load for Ascentis® Express C18 HPLC Columns?

    1 answer

    1. Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.  The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared

      Helpful?

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