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S9626

Sigma-Aldrich

Sulfatase from Helix pomatia

Type H-1, sulfatase ≥10,000 units/g solid

Sinonimo/i:

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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About This Item

Numero CAS:
9016-17-5
Classificazione EC (Enzyme Commission):
3.1.6.1 (BRENDA, IUBMB)
Numero CE:
232-772-1
Numero MDL:
MFCD00132399
Codice UNSPSC:
12352204
NACRES:
NA.54

Tipo

Type H-1

Forma fisica

powder

sulfatase activity

≥10,000 units/g solid

secondary activity

≥300 unit/mg solid β-glucuronidase (at pH 5.0)

PM

~85 kDa by gel filtration

applicazioni

diagnostic assay manufacturing

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

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Applicazioni

Sulfatase from Helix pomatia has been used in:
  • sample extraction
  • sulfatase assays
  • enzymatic hydrolysis
  • extract preparation for glucosinolate analysis

Azioni biochim/fisiol

Sulfatase from Helix pomatia facilitates the synthesis of thiohydroximates. It causes the hydrolysis of β-naphthyl sulfate much faster than a-naphthyl sulfate and possesses regiospecificity in the hydrolysis of ortho and para substituted phenyl sulfates.

Definizione di unità

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

Substrato

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H317,H334

Classi di pericolo

Resp. Sens. 1 - Skin Sens. 1

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Phytochemistry, 169, 112100-112100 (2019-11-28)
The glucosinolates (GSLs) is a well-defined group of plant metabolites characterized by having an S-β-d-glucopyrano unit anomerically connected to an O-sulfated (Z)-thiohydroximate function. After enzymatic hydrolysis, the sulfated aglucone can undergo rearrangement to an isothiocyanate, or form a nitrile or
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Vegetable consumption, including cruciferous vegetables, is protective against lung cancer, but the mechanisms are poorly understood. The purpose of this study was to investigate the effects of cruciferous vegetable consumption on the metabolism of the tobacco-specific lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)
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Glucosinolate enhancement in cabbage induced by jasmonic acid application.
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Hortscience: a Publication of the American Society For Horticultural Science Hortscience, 45, 1188-1191 (2010)
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Protocolli

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

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