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P5936

Sigma-Aldrich

Pectolyase from Aspergillus japonicus

greener alternative

suitable for plant cell culture, lyophilized powder

Sinonimo/i:

PEL1

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Classificazione EC (Enzyme Commission):
Numero MDL:
Codice UNSPSC:
10171502
NACRES:
NA.72

Forma fisica

lyophilized powder

Attività specifica

≥0.3 unit/mg solid

Caratteristiche più verdi

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

tecniche

cell culture | plant: suitable

applicazioni

agriculture

Categoria alternativa più verde

Temperatura di conservazione

2-8°C

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Descrizione generale

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Applicazioni

Pectolyase P5936 (Pel1) is a natural mixed pectolyase produced by the fungus Aspergillus japonicus used to digest components (endopolygalacturonate and pectin) of plant cell walls. Treatment of cell walls with pectolyase can be used to destablilize the cell walls for organelle isolation or to modify the elasticity of the cell walls.
Pectolyase from Aspergillus japonicas has been used to digest the siliques and also in immunofluorescence experiments.
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Altre note

Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.

Definizione di unità

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


Certificati d'analisi (COA)

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Ni C Z, et al.
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Baroux C, et al.
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Ni C, et al.
Acta Astronautica, 68(11-12), 1722-1728 (2011)
Qingxin Zhao et al.
Plant physiology, 147(4), 1874-1885 (2008-06-20)
Mature plant cell walls lose their ability to expand and become unresponsive to expansin. This phenomenon is believed to be due to cross-linking of hemicellulose, pectin, or phenolic groups in the wall. By screening various hydrolytic enzymes, we found that
Thorben Sprink et al.
International journal of molecular sciences, 22(17) (2021-09-11)
In the past, major findings in meiosis have been achieved, but questions towards the global understanding of meiosis remain concealed. In plants, one of these questions covers the need for two diverse meiotic active SPO11 proteins. In Arabidopsis and other

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