C8274
Cytohelicase from Helix pomatia
lyophilized powder
Sinonimo/i:
Snail gut enzyme
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250 MG
CHF 497.00
CHF 497.00
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Questo articolo | SAB5700806 | SAB5700703 | SAB5700796 |
|---|---|---|---|
| form lyophilized powder | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| composition Protein, 50-80% Lowry | composition - | composition - | composition - |
Application
Cytohelicase from Helix pomatia has been used:
- in the enzyme solution for preparing the cell nuclei for immuno fluorescent in situ hybridization (immunoFISH)[1]
- in the enzyme mix to analyze meiotic cells at diakinesis and later stages in anthers[2]
- in the enzyme mix for the analysis of mitosis in anthers[3]
Cytohelicase from Helix pomatia has been used to study the isolation of yeast protoplasts using various preparations of the hepato-pancreatic juice of Helix pomatia.
The enzyme from Sigma has been used for cytohelicase assay. The enzyme assay was used while studying the effects of MNN10 gene-disruption on protein secretion in Kluyveromyces lactis and Saccharomyces cerevisiae cultures.[4]
Biochem/physiol Actions
Cytohelicase from Helix pomatia is used in the preparation of synaptonemal complex spreading techniques along with bovine serum albumin. It plays an important role in the digestion of cell wall.[5][6] Cytohelicase interacts with chromatin and improves the adhesion of synaptonemal complexes to the hydrophobic plastic film.[5]
Analysis Note
Contains glucanase/laminarinase activity
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Danguole Bartkeviciūte et al.
FEMS yeast research, 4(8), 833-840 (2004-09-29)
Screening for genes affecting super-secreting phenotype of the over-secreting mutant of Kluyveromyces lactis resulted in isolation of the gene named KlMNN10, sharing high homology with Saccharomyces cerevisiae MNN10. The disruption of the KlMNN10 in Kluyveromyces lactis, as well as of
Mikhail G Divashuk et al.
PloS one, 9(1), e85118-e85118 (2014-01-28)
Hemp (Cannabis sativa L.) was karyotyped using by DAPI/C-banding staining to provide chromosome measurements, and by fluorescence in situ hybridization with probes for 45 rDNA (pTa71), 5S rDNA (pCT4.2), a subtelomeric repeat (CS-1) and the Arabidopsis telomere probes. The karyotype
Ribosomal DNA locus evolution in Nemesia: transposition rather than structural rearrangement as the key mechanism?
Datson PM and Murray BG
Chromosome Research, 14(8), 845-857 (2006)
ImmunoFISH: simultaneous visualisation of proteins and DNA sequences gives insight into meiotic processes in nuclei of grasses.
Sepsi A, et al.
Frontiers in Plant Science, 9(7), 1193-1193 (2018)
Improved method of enzyme digestion for root tip cytology.
Lattier JD, et al.
Hortscience: a Publication of the American Society For Horticultural Science Hortscience, 52(7), 1029-1032 (2017)
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