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Documenti fondamentali

L3768

Sigma-Aldrich

Lysing Enzymes from Aspergillus sp.

powder

Sinonimo/i:

Cellulase, Lysing Enzymes, Pectinase

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500 MG
CHF 344.00
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CHF 624.00

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500 MG
CHF 344.00
1 G
CHF 624.00

About This Item

Codice UNSPSC:
12352204
NACRES:
NA.54

CHF 344.00


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Origine biologica

Aspergillus niger

Stato

powder

tecniche

cell based assay: suitable

Colore

brown

Compatibilità

suitable for cell lysis

applicazioni

cell analysis

Temperatura di conservazione

2-8°C

Descrizione generale

Lysing enzymes are also known as lytic enzymes or cell wall lytic enzymes.

Applicazioni

Lysing Enzymes from Aspergillus sp. has been used to digest the cell walls to create pulse-field gel plugs.
Lysing Enzymes from Aspergillus sp. has been used:

  • to digest the cell wall of Schizosaccharomyces pombe[1]
  • to lyse the mycelium cell wall of Trichoderma harzianum[2]

Azioni biochim/fisiol

Lysing enzymes are a group of enzymes that can break down the cell walls of microorganisms. These enzymes play a crucial role in various biological processes, including cell lysis, tissue dissociation, and protein extraction. Lysing enzymes are commonly used in research, biotechnology, and clinical applications. They are especially useful in DNA extraction, where they help to release genetic material from cells by breaking down the cell walls. This enables efficient isolation and purification of DNA for downstream analyses. Cellulases are a class of enzymes that degrade cellulose, a major component of plant cell walls, into simpler sugars. These sugars can be utilized as raw materials to produce biofuels, biobased chemicals, plastics, and various other materials. Pectinases are a group of enzymes that hydrolyze pectic substances found in microorganisms and higher plants. They can break down the pectin-containing compounds synthesized by plants and microbes.

Qualità

Contains pectinase and cellulase activities.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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I clienti hanno visto anche

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Translesion synthesis polymerases (TLSPs) are non-essential error-prone enzymes that ensure cell survival by facilitating DNA replication in the presence of DNA damage. In addition to their role in bypassing lesions, TLSPs have been implicated in meiotic double-strand break repair in
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Electronic journal of Biotechnology, 10(4), 604-617 (2007)

Questions

  1. What is the working condition and concentration of this enzyme, especially for filamentous fungi cell wall digestion

    1 answer
    1. The working conditions and concentration of this enzyme is not tested. This process will have to be validated by the end-user It may be helpful to view the peer-reviewed articles:

      https://link.springer.com/article/10.1007/BF00437577
      https://www.sciencedirect.com/science/article/pii/S1087184505001052
      https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2958.2005.04827.x

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