This product is tested at pH 4.0 at 25C. The optimum pH and temperature have not been determined. However, the publication available at the link below may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5153499/#:~:text=The%20pectinase%20has%20an%20estimated,temperature%20of%2061%C2%B0C.
P4716
Pectinase from Aspergillus niger
BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)
Caratteristiche di sostenibilità
Sinonimo/i:
Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase solution from Aspergillus niger
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Cambia visualizzazione
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
5000 units | Per conoscere la disponibilità, visualizza il carrello | CHF 72.30 |
10000 units | Per conoscere la disponibilità, visualizza il carrello | CHF 133.00 |
25000 units | Per conoscere la disponibilità, visualizza il carrello | CHF 199.00 |
100000 units | Per conoscere la disponibilità, visualizza il carrello | CHF 657.00 |
Informazioni su questo articolo
Numero CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-885-6
MDL number:
Numero CE:
Specific activity:
≥5 units/mg protein (Lowry)
Biological source:
Aspergillus niger
CHF 72.30
Per conoscere la disponibilità, visualizza il carrello
Servizio Tecnico
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Permettici di aiutartibiological source
Aspergillus niger
Quality Segment
product line
BioReagent
form
aqueous glycerol solution
specific activity
≥5 units/mg protein (Lowry)
sustainability
Sustainability features
technique(s)
cell culture | plant: suitable
storage temp.
2-8°C
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.
Application
Petctinase from Aspergillus niger has been used:
- for protoplasts liberation
- in the protoplast enzyme mixture for enzyme digestion
- to conduct partial saccharification of sugars
- to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Biochem/physiol Actions
Pectolytic enzyme preparation produced from a selected strain of Aspergillus niger: contains mainly pectintranseliminase, polygalacturonase, and pectinesterase and small amounts of hemicellulases and cellulases. Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.Pectinase catalyzes the random hydrolysis of a-(1-4)-Dgalactosiduronic linkages in pectin and other galacturonans.
Physical form
Solution in 40% glycerol
Other Notes
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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Questo articolo | ||
|---|---|---|
| description BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry) | description powder, ≥750 U/g | description powder, off-white, ~0.8 U/mg |
| specific activity ≥5 units/mg protein (Lowry) | specific activity ≥750 U/g | specific activity ~0.8 U/mg |
| biological source Aspergillus niger | biological source - | biological source Aspergillus niger |
| technique(s) cell culture | plant: suitable | technique(s) - | technique(s) - |
| form aqueous glycerol solution | form powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 100 | Quality Level 100 |
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Parola di avvertenza
Danger
Codici di pericolo
Hazard Classifications
Resp. Sens. 1
Classe di stoccaggio
10 - Combustible liquids
Che succede?
WGK 3
Punto di infiammabilità (°F)
Not applicable
Punto di infiammabilità (°C)
Not applicable
DPI (dispositivi di protezione individuale)
Eyeshields, Gloves, type N95 (US)
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Hello! Is 25 degrees Celsius and pH 4.0 the optimal temperature and pH for the highest activity of this enzyme? or do I still need to check? Thanks!
1 answer-
Helpful?
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