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P3627

Sigma-Aldrich

Phosphatase, Acid from wheat germ

≥0.4 unit/mg solid

Sinonimo/i:

(Orthophosphoric-monoester phosphohydrolase [acid optimum] ), Acid Phosphatase from wheat germ

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1 G
CHF 208.00
5 G
CHF 652.00
10 G
CHF 1’120.00

CHF 208.00

Spedizione prevista il28 maggio 2025

Tutte le immagini(3)

Scegli un formato

Cambia visualizzazione
1 G
CHF 208.00
5 G
CHF 652.00
10 G
CHF 1’120.00

About This Item

Numero CAS:
9001-77-8
Classificazione EC (Enzyme Commission):
3.1.3.2 (BRENDA, IUBMB)
Numero CE:
232-630-9
Numero MDL:
MFCD00131847
Codice UNSPSC:
12352204
eCl@ss:
42010102
NACRES:
NA.54

CHF 208.00

Spedizione prevista il28 maggio 2025

Stato

powder

Attività specifica

≥0.4 unit/mg solid

PM

58 kDa by gel filtration

Temperatura di conservazione

−20°C

Stringa SMILE

O(CC(O)C)CC#C

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
GZCWLCBFPRFLKL-UHFFFAOYSA-N

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Applicazioni

Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell. Product P3627 is from wheat germ and has been used to determine the effect of phosphatase treatment on 3F3/2 staining[1].

Azioni biochim/fisiol

Plant acid phosphatase (APase) is localized in cytosol, vacuoles, and cell walls. Plant acid phosphatase is important for phosphate acquisition to mobilize organic phosphates in the soil. Phosphate starvation induces acid phosphatase generation. Acid phosphatase originates from the aleurone and scutellar tissues during germination. It hydrolyzes phytins, ATP, protein phosphates and nucleotide phosphates. It has a role in general metabolic reactions. Wheat embryo APase consists of four isoenzymes. The molecular mass of wheat germ APase is 58 kDa (gel filtration), the pH Optimum is 5.7, the pH Range is 4.0–7.0 and the optimal temperature is 45 °C.

Qualità

Contains lipase

Definizione di unità

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

Nota sulla preparazione

Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000413498
0000413498
0000391202
0000391201
0000391200

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Zuzana Novotná et al.
FEBS letters, 554(1-2), 50-54 (2003-11-05)
Phospholipase D (PLD) forms the major family of phospholipases that was first discovered and cloned in plants. In this report we have shown, for the first time, that C2 phosphatidylinositol-4,5-bisphosphate (PIP2)-dependent PLD(s) from 5 day hypocotyls of Brassica oleracea associated
H G Yu et al.
The Journal of cell biology, 145(3), 425-435 (1999-05-04)
We have identified a maize homologue of yeast MAD2, an essential component in the spindle checkpoint pathway that ensures metaphase is complete before anaphase begins. Combined immunolocalization of MAD2 and a recently cloned maize CENPC homologue indicates that MAD2 localizes
Yueming Liang et al.
Frontiers in microbiology, 11, 571209-571209 (2020-12-18)
phoD-harboring microorganisms facilitate mineralization of organic phosphorus (P), while their role in the regulation of soil P turnover under P-limited conditions in Pinus massoniana plantations is poorly understood. The aim of the present study was to investigate the effects of
Jakob Madsen Pedersen et al.
PLoS genetics, 8(12), e1003128-e1003128 (2013-01-04)
To investigate the role of DNA topoisomerases in transcription, we have studied global gene expression in Saccharomyces cerevisiae cells deficient for topoisomerases I and II and performed single-gene analyses to support our findings. The genome-wide studies show a general transcriptional
M V Stogov et al.
Klinicheskaia laboratornaia diagnostika, (11)(11), 21-22 (2013-01-12)
The article deals with the results of study of chemical composition of solitary cysts and blood serum of 27 patients. The results demonstrated that qualitative composition of f content of unicameral bone cysts is identical to chemical composition of blood
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Protocolli

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

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