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P0609

Sigma-Aldrich

Pepsin−Agarose from porcine gastric mucosa

lyophilized powder, ≥30 units/mg dry solid

Sinonimo/i:

Pepsin A

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About This Item

Classificazione EC (Enzyme Commission):
3.4.23.1 (BRENDA, IUBMB)
Numero MDL:
MFCD00081840
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

Porcine gastric mucosa

Forma fisica

lyophilized powder

Attività specifica

≥30 units/mg dry solid

PM

35 kDa

Matrice

4% cross-linked beaded agarose

N° accesso UniProt

P00791

Temperatura di conservazione

−20°C

Informazioni sul gene

pig ... LOC396892(396892)

Categorie correlate

Descrizione generale

Pepsin is an enzyme found in gastric secretion.

Applicazioni

Pepsin immobilized onto agarose beads can be used to digest and then separate out the active peptides of the glycoprotein gliadin.
Pepsin-agarose has been used as an immobilization tool to investigate the refolding ability of the pepsin enzyme. It has also been used for the preparation of F(ab′)2 fragments from rabbit IgG.
Used to produce F(ab′)2 fragments of antibodies.1

Azioni biochim/fisiol

Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. pH optimum 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH > 6.

Definizione di unità

One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)

Quantità

One gram solid swells to 5-10ml packed gel.

Stato fisico

Lyophilized powder stabilized with lactose

Inibitore

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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USP

USP

1510051

Pepsin for assay

Pepsin, Porcine Stomach Mucosa Catalyzes the hydrolysis of aminoacyl-proline to an amino acid and proline. Inhibitors include aliphatic alcohols, pepstatin A, and pH 6.0.

Sigma-Aldrich

516360

Pepsin, Porcine Stomach Mucosa

Pepsina tested according to Ph. Eur.

Sigma-Aldrich

77161

Pepsina

Pepsina powder, slightly beige, 1200-2400 U/mg

Sigma-Aldrich

77151

Pepsina

Sigma-Aldrich

Sigma-Aldrich

T4019

Trypsin−Agarose

Trypsin Agarose buffered aqueous suspension, from bovine pancreas trypsin

Sigma-Aldrich

T1763

Trypsin Agarose

Pancreatin from porcine pancreas 8 × USP specifications

Sigma-Aldrich

P7545

Pancreatin from porcine pancreas

Ludmila Tucková et al.
Journal of leukocyte biology, 71(4), 625-631 (2002-04-03)
Celiac disease, induced by dietary gluten, is characterized by mucosal atrophy and local inflammation associated with cell infiltration and activation. Unlike other food proteins, gluten and its proteolytic fragments, besides inducing a specific immune response, were shown to activate components
M Feldman et al.
Gastroenterology, 110(4), 1043-1052 (1996-04-01)
Recent studies suggesting that gastric secretion does not decrease with aging included few elderly individuals and measured only acid secretion. The aims of this study were to measure gastric acid and pepsin output in 206 health Americans (age range, 18-98
Thomas A Kraus et al.
The Journal of clinical investigation, 115(8), 2234-2243 (2005-07-26)
To explore the requirement for M cells and the Peyer's patch (PP) in induction of oral tolerance and address the potential in vivo role of intestinal epithelial cells as nonprofessional APCs, we have attempted to induce tolerance in mice with
Widya A Wahab et al.
International journal of experimental pathology, 97(4), 303-309 (2016-09-24)
Coeliac disease (CD) is an inflammatory disorder of the small intestine. It includes aberrant adaptive immunity with presentation of CD toxic gluten peptides by HLA-DQ2 or DQ8 molecules to gluten-sensitive T cells. A ω-gliadin/C-hordein peptide (QPFPQPEQPFPW) and a rye-derived secalin
Katrine Lindholm Bøgh et al.
International archives of allergy and immunology, 161(1), 21-36 (2012-12-22)
It is generally believed that protein hydrolysis in the gastrointestinal tract decreases the allergenicity of food allergens. However, it remains unknown if specific properties of digestion products determine whether a sensitisation or tolerogenic immune response will develop. We sought to
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Articoli

Antibody Fragmentation with Pepsin Digestion

Antibody fragmentation with our pepsin digestion protocol for IgG antibody fragmentation and preparation of F(ab’).

Protocolli

Enzymatic Assay of Pepsin (3.4.23.1)

This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.

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