The FBS used to prepare this product has not been heat-inactivated.
MTOXRCSUP
RPTEC Complete Supplement
sterile-filtered, suitable for cell culture, add to 500 ML of basal medium
Sinonimo/i:
Basal media supplement for huRPTEC
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Cambia visualizzazione
30 ML
CHF 123.00
About This Item
Codice UNSPSC:
12352207
NACRES:
NA.81
Prodotti consigliati
Livello qualitativo
Sterilità
sterile-filtered
Stato
liquid
tecniche
cell culture | mammalian: suitable
Temperatura di conservazione
−20°C
Descrizione generale
Sigma has developed a variety of specialized media which are intended to provide optimal performance when utilized with specific cell lines. This product consists of 30 mL of RPTEC complete media supplement which is added to basal media and other components listed below to produce 500 mL of complete media needed for optimal growth, maintenance, and culture of Sigma′s Human Renal Proximal Tubule Epithelial Cells (RPTEC). The final RPTEC complete media was developed specifically for use with Sigma′s human RPTEC control SA7K Clone cells and various knockouts (KO).
Applicazioni
RPTEC Complete Supplement has been used in SA7K cell line culture for cell characterization.[1]
Nota sulla preparazione
Prepare Complete Medium by supplementing 500 mL of MEMα (M4526) with 6.25 mL of L-Glutamine (G7513), 30 mL of RPTEC Complete Supplement (MTOXRCSUP), 0.3 mL of Gentamicin solution (G1397) and 0.03 mL of Amphotericin B solution (A2942). Complete Medium can be stored at 2–8 °C for up to 1 month. This medium is formulated for use with a 5% CO2 in air atmosphere.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Articoli
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Human renal proximal tubule epithelial cells (RPTECs) are commonly used to predict human renal drug permeability and to investigate drug efflux. We have generated transporter knockout (KO) cell lines using CompoZr® Zinc Finger Nuclease (ZFN) technology in a proprietary renal proximal tubule epithelial cell line.
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Hello, I was wondering if the FBS in the complete supplement is heat inactivated or not? This would be very helpful for us to understand our recently obtained data. Thank you in advance! With best wishes, Linda
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