LYSC9000

Lys-c (lysyl-endopeptidase), Active

from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), tag-free, solution

• Sinonimo/i: Lys-c (lysyl-endopeptidase), Active, API, Lysyl endopeptidase, Protease I
• Classificazione EC (Enzyme Commission): 3.4.21.50 (BRENDA, IUBMB)
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Origine biologica: Achromobacter lyticus
• Livello qualitativo: 200
• Ricombinante: expressed in E. coli (206-473aa)
• Grado: Proteomics Grade
• Stato: ready-to-use solution
• Concentrazione: 1 mg/mL
• pH ottimale: 8.5
• Intervallo di pH: 9.0-9.5(catalytic)
• N° accesso UniProt: P15636
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Recombinant tag-free Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.

Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.

Specificità

This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 °C.

Applicazioni

The enzyme functions optimally between 30-37 °C and suffers from degradation when subjected to temperatures above 50 °C. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 °C.

Confezionamento

1mg/ml in Plastic

Nota sulla preparazione

Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 °C.

Stoccaggio e stabilità

Store product at -20°C for up to one year. Avoid freeze/thaw cycles.

Altre note

For R&D only.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable