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ISO2

Sigma-Aldrich

Mouse Monoclonal Antibody Isotyping Reagents

sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)

Sinonimo/i:

Mouse Antibody Isotyping Kit

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1 KIT
CHF 356.00

CHF 356.00

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1 KIT
CHF 356.00

About This Item

Numero MDL:
MFCD08705607
Codice UNSPSC:
12352203
NACRES:
NA.32

CHF 356.00

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impiego

sufficient for 1000 tests (clones) (by ELISA)
 sufficient for 40 tests (clones) (by immunodiffusion, ODD)

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

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Descrizione generale

The Mouse Monoclonal Antibody Isotyping Reagents enable qualitative isotype determination of mouse monoclonal antibosies derived from hybridoma supernatant, ascites fluid or purified forms.

Specificità

ISO-2 contains 0.2 mL vials of subclass specific antibodies to all four mouse IgG subclasses, IgA, and IgM. Directions are included for using these reagents in ELISA and Ouchterlony assays to determine the subclass of monoclonal antibodies in ascites, culture supernatants, or purified preparations. Additional reagents may be required, such as conjugates for ELISA assays or agarose and buffer components for Ouchterlony assays.

Applicazioni

Determination of the subclass of a monoclonal antibody is helpful for characterization of the antibody, for choosing detection reagents, and for deciding on a purification scheme.
Mouse Monoclonal Antibody Isotyping Reagents has been used in monoclonal antibody isotype determination using enzyme-linked immunosorbent assay (ELISA).[1][2][3] It may be used in Ouchterlony immunodiffusion assays.

Caratteristiche e vantaggi

  • May be used in a variety of assay formats
  • Suitable for all antibody forms
  • Determines all mouse IgG subclasses, IgA, and IgM

Solo come componenti del kit

N° Catalogo
Descrizione
  • goat antisera to mouse IgA .2 mL
  • goat antisera to mouse IgG3 .2 mL
  • goat antisera to mouse IgG1 .2 mL
  • goat antisera to mouse IgG2a .2 mL
  • goat antisera to mouse IgG2b .2 mL
  • goat antisera to mouse IgM .2 mL

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N° Catalogo
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Codice della classe di stoccaggio

10 - Combustible liquids

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Certificati d'analisi (COA)

Lot/Batch Number
0000218263
0000218263
0000184924
0000184924
0000123341

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Protocolli

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

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